Two Metallothioneins in the Fresh-Water Fish, Crucian Carp(Carassius cuvieri) : cDNA Cloning and Assignment of Their Expression Isoforms
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概要
- 論文の詳細を見る
Two metallothionein cDNAs (MT-A and MT-B) in the fresh-water fish crucian carp (Carassius cuvieri TEMMINCK et SCHLEGEL) were cloned. Sequence analysis of both cDNAs gave the structure of a coding region corresponding to 60 amino acid residues. The homology of their deduced amino acid sequences was completely conserved at the positions of the cysteine residue, but a significant difference existed in the size of their 3'-untrans-lated regions (130 base pairs for MT-A and 280 base pairs for MT-B). Direct amino acid sequencing of the MT-II isoform purified by HPLC was accomplished for up to 30 residues and its sequence was identical to that deduced from MT-B cDNA. This is the first case in vertebrates that N-terminal methionine in crucian carp MT-II was not blocked. By northern blot analysis, basal and cadmium chloride- or dexamethasone-induced MT-B (MT-II) mRNAs were detected time dependently after treatment. On the other hand, the expression of MT-A mRNA was extremely low. These results indicate that the MT isoform II in crucian carp is coded by the MT-B gene, and that the MT-B-dominant expression of mRNA in crucian carp may be due to the difference in the 3'-untranslated regions of MT mRNAs.
- 社団法人日本薬学会の論文
- 2000-02-01
著者
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Itoh Norio
Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka Unive
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Nakanishi Tsuyoshi
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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Itoh N
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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Tanaka K
Institute Of Earth And Space Science Graduate School Of Science Osaka University
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Ren Hong-wei
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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田中 宏治
Med.safe.res.labs. Sankyo Co. Ltd. Of Shizuoka Pref.
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Tanaka K
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University:(present Office
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Itoh Norio
Dep. Toxicol. Grad. School Pharmaceut. Sci. Osaka Univ.
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Tanaka Keiichi
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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KANEKIYO Masako
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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TOMINAGA Shinpei
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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KOHROKI Junya
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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HWANG Gab-Soo
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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Hwang Gab
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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Kohroki Junya
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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Kanekiyo Masako
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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Tanaka Katsushi
Department Of Materials Science And Engineering Graduate School Of Engineering Kyoto University
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Tanaka Keiichi
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University:(present Office
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Tanaka Keiichi
Dep. Toxicol. Grad. School Pharmaceut. Sci. Osaka Univ.
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Tominaga Shinpei
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University
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Nakanishi T
Sci. Univ. Tokyo Tokyo Jpn
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Nakanishi Tsuyoshi
Department Of Toxicology Graduate School Of Pharmaceutical Sciences Osaka University:(present Office
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Nakanishi Tsuyoshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Tanaka K
Kumamoto Univ. Kumamoto Jpn
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Kojitani Takatoshi
Res. Inst. Of Pharmaceutical Sciences Musashino Univ.
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Tanaka K
Kobe‐gakuin Univ. Kobe Jpn
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Tanaka Keiichi
Department Of Emergency And Critical Care Medicine Fukuoka University Hospital
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Tanaka Keiichi
Department Of Anatomy Tottori University School Of Medicine
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Itoh Norio
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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Nakanishi Tsuyoshi
Department of Biochemistry, School of Medicine, Keio University
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