Abundance of Low Molecular Weight Phosphotyrosine Protein Phosphatase in the Nerve-Ending Fraction in the Brain
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概要
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The distribution of low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) in subcellular fractions of rat brain tissue was investigated by immunoblotting analysis using anti-LMW-PTP antibody. The enzyme was detected in the 105000 g precipitate in addition to the supernatant of brain homogenate, even after the precipitate was extensively washed, and was abundant in the particulate fraction of nerve endings. Nerve ending LMW-PTP was effectively solubilized by 1% Triton X-100 or 1% deoxycholate, though the enzyme was solubilized by thorough sonication. Two forms of LMW-PTP, designated as LMW-PTP-I and -II, were separated from the nerve ending-rich fraction by chromatofocusing. Nerve endings PTP-I and -II were different in molecular weight, isoelectric point and susceptibility to activators and inhibitors. The properties of nerve endings LMW-PTP-I and -II were similar to those of cytosolic LMW-PTP-I and -II. The abundance of LMW-PTP in nerve endings as well as in the cytosol suggests that this enzyme plays an important role in synaptic function.
- 公益社団法人日本薬学会の論文
- 1999-08-15
著者
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Shimohama Shun
Department of Neurology, Sapporo Medical University School of Medicine
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Shimohama S
Kyoto Univ. Kyoto Jpn
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Shimohama Shun
Department Of Neurology Faculty Of Medicine Kyoto University
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YAMAMOTO Ryoji
Department of Public Health, Hyogo College of Medicine
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FUJIMOTO Sadaki
Department of Environmental Biochemistry, Kyoto Pharmaceutical University
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YOSHIDA Jun-ichi
Department of Synthetic Chemistry & Biologycal Chemistry, Faculty of Engineering, Kyoto University
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Yamamoto Ryoji
Department Of Environmental Biochemistry Kyoto Pharmaceutical University
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Yoshida Jun-ichi
Department Of Environmental Biochemistry Kyoto Pharmaceutical University
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Yoshida Jun-ichi
Department Of Biological Science Faculty Of Science Kumamoto University
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Fujimoto Sadaki
Department Of Environmental Biochemistry Kyoto Pharmaceutical University
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TANINO Hiroko
Department of Environmental Biochemistry, Kyoto Pharmaceutical University
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KOBAYASHI Yumi
Department of Environmental Biochemistry, Kyoto Pharmaceutical University
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Tanino Hiroko
Department Of Environmental Biochemistry Kyoto Pharmaceutical University
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Kobayashi Yumi
Department Of Environmental Biochemistry Kyoto Pharmaceutical University
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FUJIMOTO Sadaki
Department of Biochemistry I Kyoto Pharmaceutical University:Department of III Kyoto Pharmaceutical University:Department of Pharmaceutical Chemistry II Kyoto Pharmaceutical University
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