Evaluation of the Final Deprotection System for the Solid-Phase Synthesis of Tyr(SO_3H)-Containing Peptides with 9-Fluorenylmethyloxycarbonyl (Fmoc)-Strategy and Its Application to the Synthesis of Cholecystokinin (CCK)-12
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概要
- 論文の詳細を見る
Acidolytic deprotection and cleavage conditions for an acid-labile Tyr(SO_3H)-containing peptide were systematically examined with respect to acid, temperature, and scavenger. The 90% aqueous trifluoroacetic acid (TFA)-based reagent systems (90% aqueous TFA/m-cresol and 90% aqueous TFA/m-cresol/2-methylindole) at 4℃ were found to minimize the deterioration of Tyr(SO_3Na) in the peptide. The latter deprotection/cleavage system was applied to the 9-fluorenylmethyloxycarbonyl (Fmoc)-based solid-phase synthesis of cholecystokinin (CCK)-12 on an acid-labile PAL-linked support (PAL=peptide amide linker), with Fmoc-Tyr(SO_3Na)-OH as a building block.
- 公益社団法人日本薬学会の論文
- 1993-02-15
著者
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二木 史朗
徳島大学薬学部
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二木 史朗
Faculty Of Pharmaceutical Sciences The University Of Tokushima
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北川 幸己
Faculty Of Pharmaceutical Sciences University Of Tokushima
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矢上 健
Faculty of Pharmaceutical Sciences, The University of Tokushima
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二木 史朗
Faculty Of Pharmaceutical Sciences Kyoto University
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志和 聡
Faculty of Pharmaceutical Sciences, The University of Tokushima
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志和 聡
Faculty Of Pharmaceutical Sciences The University Of Tokushima
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- Evaluation of the Final Deprotection System for the Solid-Phase Synthesis of Tyr(SO_3H)-Containing Peptides with 9-Fluorenylmethyloxycarbonyl (Fmoc)-Strategy and Its Application to the Synthesis of Cholecystokinin (CCK)-12