Purification and Characterization of Creatine Amidinohydrolase of Alcaligenes Origin
スポンサーリンク
概要
- 論文の詳細を見る
Extracellular creatine amidinohydrolase (creatinase, EC 3.5.3.3) produced by Alcaligenes sp.nov. was purified to electrophoretic homogeneity by ion exchange chromatography on diethyl-aminoethyl-cellulose, gel filtration on Sephadex G-75 and hydrophobic chromatography on phenyl-Sepharose CL-4B. The molecular weight of the enzyme was estimated to be 51000 by gel filtration on Sephadex G-200 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme showed the maximum activity at pH 8 and was stable at pH 5-9. The pI value was 4.7 as determined by isoelectric focusing. The enzyme catalyzed hydrolysis of creatine to sarcosine and urea, and the K_m and V_<max> values for creatine were 17.2 mM and 105 μmol/min/mg protein, respectively. The enzyme was markedly inactivated by p-chloromercuribenzoate (PCMB). Besides PCMB, the enzyme was inactivated by N-bromosuccinimide, Zn^<2+>, Cu^<2+> or Hg^<2+>.
- 社団法人日本薬学会の論文
- 1986-05-25
著者
-
橋本 安弘
Central Research Laboratory Kobayashi Pharmaceutical Co. Ltd.
-
中村 昭四郎
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
井上 義雄
科学警察研究所
-
Inouye Y
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
-
井上 義雄
広島大学医学部
-
井上 義雄
Institute of Pharmaceutical Science Hiroshima University School of Medicine
-
浅野 健治
Central Research Laboratory Kobayashi Pharmaceutical Co. Ltd.
-
松田 泰周
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
若松 直子
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
植出 成美
Central Research Laboratory, Kobayashi Pharmaceutical Co., Ltd.,
-
若松 直子
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
-
中村 昭四郎
Institute Of Pharmaceutical Sciences
-
中村 昭四郎
Institute Of Applied Microbiology University Of Tokyo
-
植出 成美
Central Research Laboratory Kobayashi Pharmaceutical Co. Ltd.
-
松田 泰周
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
関連論文
- A New Semi-alkaline Proteinase produced by Streptomyces cinereoruber
- Alkaline Proteinases a and b produced by Streptomyces griseoviridis
- サイクレン亜鉛錯体ダイマーの抗ウイルス(HIV)活性に対するダイマー化と錯形成の寄与(発表論文抄録(1996))
- Inhibition of Human Immunodeficiency Virus Proliferation by Macrocyclic Polyamines and Their Metal Complexes
- 8 Carbazomycin類抗生物質の単離,構造および生合成
- An Anti-inflammatory Proteinase, Kinonase AI and AIII obtained from a Streptomyces
- The Structure of Bottromycin A_2,a New Component of Bottromycins
- Novel Assay System Favorable for the Study of Cell-to-Cell Transmission of HIV-1 and Its Application to the Evaluation of Anti-HIV Drugs
- Purification and Characterization of Creatine Amidinohydrolase of Alcaligenes Origin
- Purification and Characterization of Creatinine Amidohydrolase of Alcaligenes Origin
- Structure-Activity Correlationship and Strain Specificity of Polyoxometalates in Anti-human Immunodeficiency Virus Activity
- Inhibition of Avian Myeloblastosis Virus Reverse Transcriptase by Heterocyclic Quinones : Structure-Activity Correlation
- A New Group of Antibiotics, Hydroxamic Acid Antimycotic Antibiotics. IV. Structures of Enactins Ia, Ib_1,Ib_2 and Va
- Purification and Characterization of Sarcosine Oxidase of Bacillus Origin(Biological)
- ANTIVIRAL ACTIVITY OF POLYXOMOLYBDOEUROPATE PM-104 AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
- INHIBITION OF REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS BY A HETEROPOLYOXOTUNGSTATE (PM-19)
- Suppressive Effect of Polyoxometalates on the Cytopathogenicity of Human Immunodeficiency Virus Type 1 (HIV-1) in Vitro and Their Inhibitory Activity against HIV-1 Reverse Transcriptase
- Purification and Characterization of Sarcosine Oxidase of Streptomyces Origin(Biological,Chemical)
- A New Phosphatase produced by Streptomyces yokosukanensis
- L-Glutamate Oxidase from Streptomyces violascens. I. Production, Isolation and Some Properties
- Cholesterol Esterase produced by Streptomyces lavendulae. II. Purification and Properties as a Lipolytic Enzyme
- Purification of 3β-Hydroxysteroid Oxidase of Streptomyces violascens Origin by Affinity Chromatography on Cholesterol
- Cholesterol Esterase produced by Streptomyces lavendulae
- Determination of Total and Free Cholesterol by using Cholesterol Oxidase from Streptomyces
- Determination of Serum Glutamate Oxaloacetate Transaminase and Glutamate Pyruvate Transaminase by Using L-Glutamate Oxidase
- L-Glutamate Oxidase from Streptomyces violascens. II. Properties
- 新抗生物質Azomycinの構造
- ストレプトニグリン誘導体の生物学的性質 : 1. 抗菌及び殺細胞活性(発表論文抄録(1985年))
- Affinity Chromatography of Alkaline Proteinase S on N-Carbobenzoxyglycylleucylaminohexyl-Sepharose
- 3,3'-(フェニルホスホリル) ビス(1,3-チアゾリジン-2-チオン)を用いるストレプトニグリン2-アミド誘導体の合成(発表論文抄録(1986年))
- Affinity Chromatography of Neutral Metalloendopeptidase Produced by Streptomyces mauvecolor on N-Benzyloxycarbonylglycyl-D-leucylaminohexyl-Sepharose
- Affinity Chromatography of Neutral Metalloendopeptidase Produced by Streptomyces griseoruber on N-Benzyloxycarbonylglycylleucyl-aminohexylamino-Sepharose
- Affinity Chromatography of Alkinonase A on N-Carbobenzoxy-Glycyl-Leucyl-Aminohexyl-Sepharose
- Production of Oleanene Triterpenes by Streptomyces
- Purification and Characterization of Extracellular 3β-Hydroxysteroid Oxidase produced by Streptoverticillium cholesterolicum
- カブトガニの抗菌性ペプチドpolyphemusinIIアナログの抗HIV活性
- A Method to Screen Anticholesterol Substances produced by Microbes and a New Cholesterol Oxidase produced by Streptomyces violascens
- Structure of Amidinomycin.
- Studies on Structure of Griseolutein-B, a Streptomyces Antibiotic. III. The Complete Structure.
- Studies on the Structure of Griseolutein-B, a Streptomyces Antibiotic. II. Decarboxylation and Periodic Acid Oxidation.
- Studies on Structure of Griseolutein B, a Streptomyces Antibiotic. I. Characterization and Degradation.