Determination of Total and Free Cholesterol by using Cholesterol Oxidase from Streptomyces
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概要
- 論文の詳細を見る
Total and free serum cholesterol was determined by using a new cholesterol oxidase preparation obtained from Streptomyces. Cholesterol esters were hydrolyzed by cholesterol esterase, and free cholesterol was oxidized to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively coupled with 4-aminoantipyrine and phenol to form the quinoneimine dye. The produced color was measured by its absorption at 500nm. The results of serum cholesterol determination correlate well with those obtained by Zak-Henly and Kiliani method. Moreover, this enzymatic method is simpler and more sensitive than the conventional method.
- 公益社団法人日本薬学会の論文
- 1977-06-25
著者
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中村 昭四郎
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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鈴木 肇
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
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石丸 くるみ
広島大薬
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中村 昭四郎
Institute Of Pharmaceutical Sciences
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中村 昭四郎
Institute Of Applied Microbiology University Of Tokyo
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