L-Glutamate Oxidase from Streptomyces violascens. II. Properties
スポンサーリンク
概要
- 論文の詳細を見る
The properties of L-glutamate oxidase, a novel L-glutamic acid oxidizing enzyme from Streptomyces violascens H82-N-SY7 were examined. The enzyme showed absorption maxima at 280,390 and 470 nm and a marked shoulder at 490 nm, and contained 1 mol of flavin-adenine dinucleotide (FAD) per mol of enzyme. Enzyme activity was inhibited by Ag^+, Hg^<2+>, p-chloromercuribenzoate (PCMB) and N-bromosuccinimide. The enzyme catalyzed the oxidation of L-glutamic acid using molecular oxygen as a primary electron acceptor and produced α-ketoglutaric acid, NH_3 and hydrogen peroxide according to the following schema : L-glutamic acid+H_2O+O_2→α-ketoglutaric acid+NH_3+H_2O_2. L-Glutamate oxidase was used in a specific and sensitive determination procedure for L-glutamic acid.
- 公益社団法人日本薬学会の論文
- 1983-10-25
著者
-
中村 昭四郎
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
松崎 明紀
Institute of Microbial Chemistry
-
近藤 久雄
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
-
亀井 敏夫
Institute of Chemical Pharmacology, Banyu Pharmaceutical Co., Ltd.
-
浅野 和子
Institute of Chemical Pharmacology, Banyu Pharmaceutical Co., Ltd.
-
浅野 和子
Laboratory Of Microbiology Meguro Research Laboratories Banyu Pharmaceutical Co. Ltd.
-
中村 昭四郎
Institute Of Pharmaceutical Sciences
-
中村 昭四郎
Institute Of Applied Microbiology University Of Tokyo
-
松崎 明紀
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
関連論文
- A New Semi-alkaline Proteinase produced by Streptomyces cinereoruber
- Alkaline Proteinases a and b produced by Streptomyces griseoviridis
- 8 Carbazomycin類抗生物質の単離,構造および生合成
- モンゴル・ウランバートル周辺の活断層
- Alkinonase A and AF, New Alkaline Proteinases produced by Streptomyces violaceorectus
- Inhibition of Dopamine β-Hydroxylase by 5-Alkylpicolinic Acid and Their Hypotensive Effects
- An Anti-inflammatory Proteinase, Kinonase AI and AIII obtained from a Streptomyces
- The Structure of Bottromycin A_2,a New Component of Bottromycins
- Purification and Characterization of Creatine Amidinohydrolase of Alcaligenes Origin
- Purification and Characterization of Creatinine Amidohydrolase of Alcaligenes Origin
- Inhibition of Avian Myeloblastosis Virus Reverse Transcriptase by Heterocyclic Quinones : Structure-Activity Correlation
- A New Group of Antibiotics, Hydroxamic Acid Antimycotic Antibiotics. IV. Structures of Enactins Ia, Ib_1,Ib_2 and Va
- Purification and Characterization of Sarcosine Oxidase of Bacillus Origin(Biological)
- ANTIVIRAL ACTIVITY OF POLYXOMOLYBDOEUROPATE PM-104 AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
- INHIBITION OF REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS BY A HETEROPOLYOXOTUNGSTATE (PM-19)
- Suppressive Effect of Polyoxometalates on the Cytopathogenicity of Human Immunodeficiency Virus Type 1 (HIV-1) in Vitro and Their Inhibitory Activity against HIV-1 Reverse Transcriptase
- Purification and Characterization of Sarcosine Oxidase of Streptomyces Origin(Biological,Chemical)
- A New Phosphatase produced by Streptomyces yokosukanensis
- L-Glutamate Oxidase from Streptomyces violascens. I. Production, Isolation and Some Properties
- Cholesterol Esterase produced by Streptomyces lavendulae. II. Purification and Properties as a Lipolytic Enzyme
- Purification of 3β-Hydroxysteroid Oxidase of Streptomyces violascens Origin by Affinity Chromatography on Cholesterol
- Cholesterol Esterase produced by Streptomyces lavendulae
- Determination of Total and Free Cholesterol by using Cholesterol Oxidase from Streptomyces
- Determination of Serum Glutamate Oxaloacetate Transaminase and Glutamate Pyruvate Transaminase by Using L-Glutamate Oxidase
- L-Glutamate Oxidase from Streptomyces violascens. II. Properties
- 新抗生物質Azomycinの構造
- Affinity Chromatography of Alkaline Proteinase S on N-Carbobenzoxyglycylleucylaminohexyl-Sepharose
- 3,3'-(フェニルホスホリル) ビス(1,3-チアゾリジン-2-チオン)を用いるストレプトニグリン2-アミド誘導体の合成(発表論文抄録(1986年))
- Affinity Chromatography of Neutral Metalloendopeptidase Produced by Streptomyces mauvecolor on N-Benzyloxycarbonylglycyl-D-leucylaminohexyl-Sepharose
- Affinity Chromatography of Neutral Metalloendopeptidase Produced by Streptomyces griseoruber on N-Benzyloxycarbonylglycylleucyl-aminohexylamino-Sepharose
- Affinity Chromatography of Alkinonase A on N-Carbobenzoxy-Glycyl-Leucyl-Aminohexyl-Sepharose
- Production of Oleanene Triterpenes by Streptomyces
- Purification and Characterization of Extracellular 3β-Hydroxysteroid Oxidase produced by Streptoverticillium cholesterolicum
- A Method to Screen Anticholesterol Substances produced by Microbes and a New Cholesterol Oxidase produced by Streptomyces violascens
- Structure of Amidinomycin.
- Studies on Structure of Griseolutein-B, a Streptomyces Antibiotic. III. The Complete Structure.
- Studies on the Structure of Griseolutein-B, a Streptomyces Antibiotic. II. Decarboxylation and Periodic Acid Oxidation.
- Studies on Structure of Griseolutein B, a Streptomyces Antibiotic. I. Characterization and Degradation.