Purification of 3β-Hydroxysteroid Oxidase of Streptomyces violascens Origin by Affinity Chromatography on Cholesterol
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概要
- 論文の詳細を見る
Cholesterol could be used to adsorb the 3β-hydroxysteroid oxidase of Streptomyces violascens origin from a crude enzyme solution, and the adsorbed enzyme could be eluted with a suitable detergent such as Triton X-100. The enzyme was purified by ammonium sulfate precipitation and affinity chromatography on cholesterol with a recovery of 79% from the culture filtrate. The purified enzyme was detected as a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight of the enzyme was 61000 by SDS-polyacrylamide gel electrophoresis. The purified enzyme exhibited a characteristic spectrum of flavoenzyme. The flavin moiety of the enzyme was isolated and identified as flavin adenine dinucleotide.
- 公益社団法人日本薬学会の論文
- 1978-09-25
著者
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中村 昭四郎
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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松崎 明紀
Institute of Microbial Chemistry
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鈴木 肇
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
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中村 昭四郎
Institute Of Pharmaceutical Sciences
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中村 昭四郎
Institute Of Applied Microbiology University Of Tokyo
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松崎 明紀
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
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