Cholesterol Esterase produced by Streptomyces lavendulae. II. Purification and Properties as a Lipolytic Enzyme
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概要
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Cholesterol esterase was purified about 85 times from the broth filtrate of Streptomyces lavendulae by procedures involving precipitation with ammonium sulfate, reprecipitation with acetone, affinity chromatography on palmitoyl cellulose, and ion exchange chromatography on DEAE-cellulose. The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis and SDS-polyacrylamide gel electrophoresis, and the specific activity of the enzyme was 36.3 U/mg. The molecular weight of the enzyme was 12000 as determined by SDS-polyacrylamide gel electrophoresis. The enzyme showed different properties from other cholesterol esterases as regards its affinity for cholesterol.
- 公益社団法人日本薬学会の論文
- 1979-07-25
著者
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中村 昭四郎
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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松崎 明紀
Institute of Microbial Chemistry
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鈴木 肇
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
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浅野 和子
Laboratory Of Microbiology Meguro Research Laboratories Banyu Pharmaceutical Co. Ltd.
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中村 昭四郎
Institute Of Pharmaceutical Sciences
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中村 昭四郎
Institute Of Applied Microbiology University Of Tokyo
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松崎 明紀
Institute Of Chemical Pharmacology Banyu Pharmaceutical Co. Ltd.
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