L-Glutamate Oxidase from Streptomyces violascens. I. Production, Isolation and Some Properties

概要

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L-Glutamate oxidase, a novel L-glutamic acid oxidizing enzyme, has been found in the culture broth of Streptomyces violascens. The enzyme has been purified 914-fold by precipitation with ammonium sulfate, affinity chromatography on L-glutamic acid-Sepharose 6B and L-glutamine-Sepharose 6B, hydroxyapatite chromatography and gel filtration on Sephadex G-100. The purified enzyme showed a single band on polyacryl-amide disc gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The molecular weight of the enzyme was estimated to be about 60000. The purified enzyme exhibited a characteristic flavoprotein spectrum. The enzyme catalyzed the oxidation of L-glutamic acid and L-glutamine without a requirement for any exogenous cofactor.
公益社団法人日本薬学会の論文
1983-04-25