培養 HeLa 細胞の形態に及ぼす代謝阻害薬の影響

概要

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A contrivance, named modified panel method, had been developed for a screening tool for the purpose of the bioassay of carcinogens and anticarcinogens. In this study the growth inhibition and morphological changes which were brought to HeLa cells by three compounds of known mechanism of action, hydroxyurea, proflavin hemisulfate and cycloheximide, were observed with the above method. Then it was tried to measure areas of cytoplasm, nucleus and nucleolus of tested cells with digitizing system of personal computer, and to represent morphological changes by quantitative difference found in the measurement. The results show that these trials are useful for examination of the correlation between changes in morphology and in metabolism of cells affected by bioactive substances and for classification of those changes.
九州歯科学会の論文
1986-12-25