培養 HeLa 細胞に及ぼすホルマリンクレゾールの影響

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Formalincresol (FC), a mixture of the same quantity each of formalin and cresol, was tested for its cytotoxic effect on HeLa cell tissue culture system in comparison with formalin and cresol. In the first study, the cells which had grown to monolayers were exposed to FC and observed through the phase contrast microscope for 24 hours. The degree of morphological degeneration was estimated on a scale ranging 0 (no change) through 4 (granulation and rounding up of cells). In the second study, FC, formalin or cresol was added to the suspended cells. At the end of 48-hour cultivation, the degree of inhibitory effect of the drug on cell-growth was estimated on a scale ranging 0 (no inhibition) through 4 (no growth), and then cells were stained with heamatoxylin and eosin for the examination. In the third study, the viable cells were counted up after being treated in the same way as in the second study. The results were as follows : 1. No significance of toxicity was perceived at 5 μg/ml and a slight reversible shrinkage of cells of established monolayer occurred at 10 μg/ml of FC. 2. The cells of established monolayer were brought to the most conspicuous and irreversible change in morphology when exposed to FC in the concentration of 50 to 100μg/ml, and nearly fixed at and over 500 μg/ml. 3. Concentration of drug causing 50% inhibition of adhering of suspended cells was 12.5 μg/ml for FC, 9.0 μg/ml for formalin and 60 μg/ml for cresol. 4. There was more similarity in the morphological changes caused by FC and cresol than by FC and formalin.
1986-12-25

培養 HeLa 細胞に及ぼすホルマリンクレゾールの影響

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