ブラシノステロイドの液体クロマトグラフィー : 質量分析(<特集>質量分析法の応用技術)

概要

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A microanalytical LC/MS method for the determination of brassinosteroids as their boronates has been developed. Under reversed-phase liquid chromatographic conditions the brassinolide boronates, formed by naphthaleneboronic acid, m-aminophenylboronic acid or dansylaminophenylboronic acid, have been analyzed using atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) methods. The effects of additives to the liquid chromatographic mobile phase on the sensitivity for detection of the boronate were examined. A detection limit of 25 picogram (signal-to-noise ratio 3) of brassinolide as naphthaleneboronate was attained in the selected-ion monitoring (SIM) mode with electrospray ionization, and that of 2.5 picogram (signal-to-noise ratio 4) for the naphthaleneboronate derivative was attained in the multiple reaction monitoring (MRM) mode with ESI. The LC/MS analysis of these boronate derivatives could be very useful for trace analysis of brassinosteroids.
社団法人日本分析化学会の論文
1996-06-05