含シアン廃水の生物学的処理に関する研究 : (第1報)シアンを窒素源としたシアン耐性菌の培養について

概要

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A strain of cyanide-resistant bacteria was isolated from soil which had been polluted with the waste water of an electro-plating plant, and its growth condition in a medium containing cyanide as the nitrogen source was studied.(1) The cyanide-resistant bacterium grew well in the alkaline range (the optimum initial pH was 10.2), and in medium containing 400-500 ppm cyanide, but the growth was gradually inhibited by increasing the cyanide concentration.(2) Other than the cyanide, the bacterium also utilized urea, ammoniacal nitrogen, and peptone, while it could not utilize nitrous, nitrate, or nitrile nitrogens.(3) Glucose, fructose, mannose, and galactose were utilized as carbon sources.(4) Thiamine hydrochloride in the medium (100-400 μg/dl) accelerated the growth. Riboflavin also accelerated growth at a concentration up to 100μg/dl, but the growth was inhibited at more than 150μg/dl.(5) Addition of glutathione and cysteine also accelerated the growth.(6) The bacterium showed several peaks of the activity for cyanide degradation at pH 5.3,7.0,and at 10.0. At pH 10.0,degradation activity was the highest in the cells of the exponential growth phase, and lower in the subsequent growth phase.
社団法人日本生物工学会の論文
1972-05-25