炭酸固定発酵に関する研究 : (第13報) スエヒロタケによるL-Malate の副産物としてのセルラーゼ

概要

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The L-malic acid production (up to 90% of sugar consumed) of Schizophyllum commune Fries IFO No. 4928 in such basal medium as shown in Table 1 was found by Sei.Tachibana in 1960. It has already been revealed by the authors that L-malic acid was biosynthesized through a CO_2-fixing process by aphoenolpyruvic carboxytransphosphorylase-like enzyme. The production of cellulase from the strain is investigated in the paper. Some activities of CMC-ase were observed in a culture filtrate of L-malic acid fermentation. The effects on the CMC-liquefying, CMC-saccharifying, filter paper-disintegrating and filter paper-saccharifying activities were observed by the addition of CMC, cellulose powder, filter paper, CM-cellulose and cotton one by one the basal medium.Filter paper was found to be the most powerful inducer for both formations of CMC-ase and filter paper degrading enzyme. CM-cellulose seemed to be more specific for the inducible for formation of filter paper degrading enzyme than CMC. Any one of the inducers could not inhibit the yield of L-malate production. The peaks of the cellulase formation coincided with the peaks of L-malate production and of glucose comsumption, as shown in Fig. 5.Therefore, it was suggested that the simultaneous production of cellulase and L-malic acid in the CO_2-fixing process using Schizophyllum commune Fries IFO No. 4928 was possible.
社団法人日本生物工学会の論文
1965-10-25