播磨灘におけるビタミンB_<12>濃度の変動

概要

論文の詳細を見る
Studies were made of the variations of vitamin B_<12> concentration in the Sea of Harima.1) The amount of vitamin B_<12> in the seawater was measured by microbioassay with Lactobacillus leichmannii after extracting vitamin B_<12> with benzylalcohol. This method promised a high recovery rate. Determination was set for a sensitivity level of 2 pg/ml.2) Bioautograph records suggested that what was measured as vitamin B_<12> in seawater was the cobalamin derivative.3) Vitamin B_<12> concentrations in the seawater, river water, industrial wastewater treated with activated sludge, and the sediments on the sea bottom were respectively : 0-25 pg/ml, 2-10 pg/ml, about 10 pg/ml and 0.7-1.4 ng/g-sediment.4) A high concentration of vitamin B_<12> was often found in warm water, but no specific pattern was found either in vertical or horizontal distribution. Cold water contained low concentrations of vitamin B_<12>. A significant difference in vitamin B_<12> concentration was found between surface water and bottom layer water.5) Vitamin B_<12> in seawater decreased in the photosynthesis process of phytoplankton and increased in the mineralization processes of organic compounds.
1984-07-25