沢庵漬着色剤の変色現象に関する研究 : (第8報)DNNS (2,4-dinitro-1-naphothol-7-sulfonic acid)還元酵素系実験法の検討

概要

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Prior to the investigation into the properties of DNNS (2,4-dinitro-1-naphthol-7-sulfonic acid) reductase system, several conditions necessary for enzymatic experiments with cell suspensions were established as follows : 1. Extinction cofficients of centrifuged supernatants from reaction mixtures were measured at 486mμ, and the reductase activities were expressed in terms of the above coefficients.2. On the other hand, dehydrogenase activities were expressed by time values for the reduction of methylene blue or by oxygen uptake with Warburg manometry.3. In experiments for the reduction of DNNS, cells were obtained from cultures incubated for 3〜4 days at 25℃.
社団法人日本生物工学会の論文
1962-03-15