Aspergillus oryzaeの連続培養によるプロテアーゼの生産 : 菌糸の形態変化に伴う撹拌に対する抵抗力の変化

概要

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The effects of agitation rete and oxygen content in the supplied gas on protease production in the continuous culture of Aspergillus oryzae were investigated. 1.A difference in the morphology of the mycelia was found between the start-up and steady states in the continuous culture. The resistance of mycelia against shear force during the steady state (thin and short) was stronger than that in the start-up state (thic and long). The degree of damage caused by the shear force increased with increasing oxygen content in the supplied gas in the both types. 2.The thin and short form of mycelia could be obtained by maintaining the dry weight of the mycelia at about 7 mg/ml by controlling the medium content, keeping the agitation rate under the critical rate, and suplying enough oxygen to produce protese during the start-up state in the continuous culture. 3.By regarding the impeller tip velocity during the start-up and the dissoloved oxygen concentration in the steady state as the scale up indexes, protease activity could be increased to 1,800 PU/ml in a 200 l continuous culture.
社団法人日本生物工学会の論文
1991-11-25