Tissue-Specific Regulation of 4E-BP1 and S6K1 Phosphorylation by α-Ketoisocaproate
スポンサーリンク
概要
- 論文の詳細を見る
The indispensable branched-chain amino acid leucine acts as a key regulator of mRNA translation by modulating the phosphorylation of proteins that represent important control points in translation initiation, including the translational represser, eukaryotic initiation factor (eIF) 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (S6K1). In the current study, we compared the effects of L- and D-enantiomers of leucine on the phosphorylation of 4E-BP1 and S6K1. We also assessed whether leucine itself or its metabolite, α-ketoisocaproate (α-KIC), mediates the effects of leucine. Food-deprived (18 h) rats were orally administered 135 mg/100 g body weight L-leucine, D-leucine or α-KIC and were sacrificed after 1 h. L-Leucine administration had an obvious stimulatory effect on the phosphorylation of 4E-BP1 and S6K1 in both skeletal muscle and liver while D-leucine was much less effective, indicating that the effect of leucine is stereospecific. Oral administration of α-KIC mimicked the stimulatory effect of L-leucine in skeletal muscle. In contrast to skeletal muscle, provision of α-KIC was significantly less effective than L-leucine in the liver. The results showing that the efficacy of L-leucine and α-KIC in stimulating phosphorylation of S6K1 and 4E-BP1 is equivalent in skeletal muscle, may be explained by the conversion of α-KIC to L-leucine.
- 日本ビタミン学会の論文
著者
-
YOSHIZAWA Fumiaki
Department of Science of Living, Morioka Junior College, Iwate Prefectural University
-
Yoshizawa Fumiaki
宇都宮大学
-
Yoshizawa Fumiaki
Department Of Animal Science Utsunomiya University
-
Yoshizawa Fumiaki
Department Of Animal Science Faculty Of Agriculture Utsunomiya University
-
NAGASAWA Takashi
Department of Agro-bioscience, Iwate University
-
SEKIZAWA Haruhito
Department of Animal Science, Utsunomiya University
-
HIRAYAMA Sachiyo
Department of Animal Science, Utsunomiya University
-
YAMAZAKI Yasuhiro
Department of Animal Science, Utsunomiya University
-
SUGIHARA Kunio
Department of Animal Science, Utsunomiya University
-
Sugahara K
Department Of Animal Science Utsunomiya University
-
Sekizawa Haruhito
Department Of Animal Science Utsunomiya University
-
Sekizawa Haruhito
宇都宮大学
-
Nagasawa Takashi
Depatment Of Biological Chemistry And Food Sciences Faculty Of Agriculture Iwate University
-
Nagasawa Takashi
岩手大学 連合農学研究科
-
Hirayama Sachiyo
Department Of Animal Science Utsunomiya University
-
Hirayama Sachiyo
宇都宮大学
-
Sugahara Kunio
宇都宮大学 農学部
-
Nagasawa Takashi
Department Of Agro-bioscience Iwate University
-
Nagasawa Takashi
Food And Health Science Faculty Of Agriculture Iwate University
-
Yoshizawa Fumiaki
Department Of Animal Science Division Of Bio-productive Science Faculty Of Agriculture Utsunomiya Un
-
Sugihara Kunio
Department Of Animal Science Utsunomiya University
-
Yamazaki Yasuhiro
Department Of Animal Science Utsunomiya University
-
Nagasawa T
Depatment Of Biological Chemistry And Food Sciences Faculty Of Agriculture Iwate University
-
NAGASAWA Takashi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
-
HIRAYAMA Sachiyo
Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
関連論文
- Collaborative Research on Adhesion Strength of Thermal Barrier Coatings in JSMS
- Oxidative Stress Induces Phosphoenolpyruvate Carboxykinase Expression in H4IIE Cells
- Elongation Factor 2 in the Liver and Skeletal Muscle of Mice is Decreased by Starvation
- Insulin-stimulated Polypeptide Chain Elongation in the Soleus Muscle of Mice
- Rapid Dephosphorylation of eIF4E by Dietary Protein in the Skeletal Muscle and Liver of Food-deprived Rats
- Stimulative Effect of Dietary Protein on the Phosphorylation of p70 S6 Kinase in the Skeletal Muscle and Liver of Food-deprived Rats
- Tissue-Specific Regulation of 4E-BP1 and S6K1 Phosphorylation by α-Ketoisocaproate
- Oral Administration of Leucine Stimulates Phosphorylation of 4E-BP1 and S6K1 in Skeletal Muscle but Not in Liver of Diabetic Rats
- Time Course of Leucine-induced 4E-BP1 and S6K1 Phosphorylation in the Liver and Skeletal Muscle of Rats
- Supplementation with Dietary Leucine to a Protein-Deficient Diet Suppresses Myofibrillar Protein Degradation in Rats
- Effects of Dietary Protein of Korean Foxtail Millet on Plasma Adiponectin, HDL-Cholesterol, and Insulin Levels in Genetically Type 2 Diabetic Mice
- Suppression of Myofibrillar Protein Degradation after Refeeding in Young and Adult Mice
- Myofibrillar Protein Catabolism is Rapidly Suppressed Following Protein Feeding
- Plasma N^τ-Methylhistidine Concentration Is a Sensitive Index of Myofibrillar Protein Degradation during Starvation in Rats
- Measurement of the Rate of Myofibrillar Protein Degradation Using the Arteriovenous Difference in Plasma 3-Methylhistidine Concentration of Rats
- Dopamine Release in the Ventromedial Hypothalamus of Growing Chickens Decreases when They are Fed a Lysine Devoid Diet
- Enhancing Effect of Dietary Vinegar on the Intestinal Absorption of Calcium in Ovariectomized Rats
- The Tyrosine Kinase Activity of the Chicken Insulin Receptor Is Similar to That of the Human Insulin Receptor
- Nutritional Regulation of Insulin-like Growth Factor-I Receptor mRNA Levels in Growing Chickens
- Effect of Starvation on the Plasma N^τ-Methylhistidine Concentration in Goats
- Regulation of Hydroxyindole-0-methyltransferase Gene Expression in Japanese Quail (Coturnix coturnix japonica)(Biochemistry & Molecular Biology)
- Feeding a Lysine-free Diet Decreases Food Intake without Changes in Hypothalamic Monoamine Concentrations in Growing Chicks
- Feeding a Lysine-free Diet Decreases Food Intake without Changes in Hypothalamic Monoamine Concentrations in Growing Chicks
- Decrease of the Obese Gene Expression in Bovine Subcutaneous Adipose Tissue by Fasting
- 烏骨鶏における松果体中メラトニン含量のサ-カディアンリズム
- Effects of Dietary Korean Proso-Millet Protein on Plasma Adiponectin, HDL Cholesterol, Insulin Levels, and Gene Expression in Obese Type 2 Diabetic Mice
- Effect of Quality and Quantity of Dietary Protein on 4E-BP1 and S6K1 Phosphorylation of Brains in Aged Rats
- Effects of Dietary Protein of Proso Millet on Liver Injury Induced by D-galactosamine in Rats(Food & Nutrition Science)
- A Chemokine, SDF-1/PBSF, and Its Receptor, CXC Chemokine Receptor 4, as Mediators of Hematopoiesis
- L.S.3 Physiological functions of branched-chain amino acids (BCAA)(The Proceedings of the 15th Annual Meetings of Japan Society of Exercise and Sports Physiology July 26-27, (Hirosaki))
- Induction of Oxidatively Modified Proteins in Skeletal Muscle by Electrical Stimulation and Its Suppression by Dietary Supplementation of (-)-Epigallocatechin Gallate
- Isolation and Characterization of a 41 kDa Sericin from the Wild Silkmoth Antheraea yamamai
- High Molecular Weight Protease in Rumen Ciliate Protozoa
- Effects of Dietary Korean Proso-Millet Protein on Plasma Adiponectin, HDL Cholesterol, Insulin Levels, and Gene Expression in Obese Type 2 Diabetic Mice
- Dietary Japanese Millet Protein Ameliorates Plasma Levels of Adiponectin, Glucose, and Lipids in Type 2 Diabetic Mice
- Ethionine-Induced ATP Depletion Represses mTOR Signaling in the Absence of Increases in AMP-Activated Protein Kinase Activity in the Rat Liver
- Polypeptide Compositions and NH_2-terminal Amino Acid Sequences of Proteins in Foxtail and Proso Millets
- Secretion of Human Atrial Natriuretic Peptide in Response to Atrial Pacing and Its Function in Patients with Ventricular Septal Defect
- New Therapeutic Strategy for Amino Acid Medicine : Notable Functions of Branched Chain Amino Acids as Biological Regulators
- Oral Administration of Leucine Stimulates Phosphorylation of 4E-BP1 and S6K1 in Skeletal Muscle but Not in Liver of Diabetic Rats.
- The Effect of Insulin on Myofibrillar Protein Degradation in Normal and Streptozotocin-induced Diabetic Rats Measured by the Rate of Nτ-Methylhistidine Release from Perfused Hindquarters
- Heterogenous Degradation of Myofibrillar Proteins