Oral Administration of Leucine Stimulates Phosphorylation of 4E-BP1 and S6K1 in Skeletal Muscle but Not in Liver of Diabetic Rats.
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概要
- 論文の詳細を見る
Leucine performs a signaling role to enhance protein synthesis by phosphorylating eukaryotic initiation factor (elF) 4E-binding protein 1 (4E-BP1) and 70-kDa riboso-mal protein S6 kinase (S6K1), two key regulatory proteins involved in the initiation of mRNA translation. The purpose of the current study was to assess whether the phosphorylation of 4E-BP1 and S6K1 was increased in skeletal muscle and liver by an oral administration of Leucine to diabetic rats and to determine the in vivo contribution of insulin to a leucine-dependent induction of 4E-BP 1 and S 6K 1 phosphorylation. Food-deprived (18 h) normal and diabetic rats were orally administered 135mg/ 100g body weight L-leucine and sacrificed at 1 h after administration. Leucine administration resulted in enhanced phosphorylation of 4E-BP 1 and S 6K 1 in skeletal muscle and in liver of nondiabetic rats. The stimulatory action of leucine on the phosphorylation of 4E-BP 1 and S 6K 1 in skeletal muscle was not abolished in rats with streptozotocin-induced diabetes. In contrast, leucine administration did not stimulate the phosphorylation of 4E-BP1 and S6K1 in the liver of diabetic rats. These findings suggest that in skeletal muscle, leucine functions as a nutritional signaling molecule that independently regulates the phosphorylation states of 4E-BP 1 and S6K1. In contrast to skeletal muscle, insulin is essential in mediating the leucine-dependent induction of 4E-BP1 and S6K1 phosphorylation in liver.
- 財団法人 学会誌刊行センターの論文
著者
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Sekizawa Haruhito
Department Of Animal Science Utsunomiya University
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Hirayama Sachiyo
Department Of Animal Science Utsunomiya University
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Sugahara Kunio
Department Of Animal Science Division Of Bio-productive Science Faculty Of Agriculture Utsunomiya Un
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Nagasawa Takashi
Department Of Agro-bioscience Iwate University
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Yoshizawa Fumiaki
Department Of Animal Science Division Of Bio-productive Science Faculty Of Agriculture Utsunomiya Un
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YOSHIZAWA Fumiaki
Department of Animal Science, Faculty of Agriculture, Utsunomiya University,
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NAGASAWA Takashi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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HIRAYAMA Sachiyo
Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
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NAGASAWA Takashi
Department of Animal Science, Faculty of Agriculture, Utsunomiya University,
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