Purification and Properties of Thiosulfate Dehydrogenase from Acidithiobacillus thiooxidans JCM7814

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概要

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• A key enzyme of the thiosulfate oxidation pathway in Acidithiobacillus thiooxidans JCM7814 was investigated. As a result of assaying the enzymatic activities of thiosulfate dehydrogenase, rhodanese, and thiosulfate reductase at 5.5 of intracellular pH, the activity of thiosulfate dehydrogenase was measured as the key enzyme. The thiosulfate dehydrogenase of A. thiooxidans JCM7814 was purified using three chromatographies. The purified sample was electrophoretically homogeneous. The molecular mass of the enzyme was 27.9 kDa and it was a monomer. This enzyme had cytochrome c. The optimum pH and temperature of this enzyme were 3.5 and 35℃. The enzyme was stable in the pH range from 5 to 7,and it was stable up to 45℃. The isoelectric point of the enzyme was 8.9. This enzyme reacted with thiosulfate as a substrate. The K_m was 0.81mM.

• 社団法人日本農芸化学会の論文
• 2001-01-23

著者

• NAKAMURA Kazuo

  Department of Management and Information Systems Science Nagaoka University of Technology

• Yoshikawa Hiroshi

  Department Of Biopharmaceutics Kyoto Pharmaceutical University:exploratory Development Laboratories

• Nakamura K

  Kyoto Univ. Uji Jpn

• AMANO YOSHIFUMI

  Department of Fermentation Technology, Faculty of Engineering, Yamanashi University

• Yoshikawa H

  Graduate School Of Veterinary Medicine Azabu University

• NAKAMURA Maya

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Nakamura Maya

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Amano Y

  Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin

• Amano Yoshifumi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Nakamura Kazuo

  Department Of Clinical Pharmacy And Pharmacology Graduate School Of Medical And Dental Sciences Kago

• Nakamura Kazuo

  Department Of Biochemistry Kitasato University School Of Medicine

• Yoshikawa Hiroshi

  Department of Applied Physics, Handai Frontier Research Center, and Venture Business Laboratory of Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan

• Nakamura Kazuo

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

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