Determination of Free Sulfur Dioxide in Wine by Using a Biosensor Based on a Glass Electrode
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概要
- 論文の詳細を見る
A biosensor based on a glass electrode was developed to determine free sulfur dioxide in wine. The biosensor consists of a microbial membrane of Thiobacillus thiooxidans JCM7814 and a flat glass electrode. A porous gas-permeable membrane was incorporated in the biosensor system to separate free sulfur dioxide from bound sulfur dioxide and to avoid the buffer action of the sample solution. This biosensor gave a linear relationship between the pH decrease and concentration of sulfur dioxide up to 50mg/liter with a response time of 20min. The limit of detection for sulfur dioxide was 5.0mg/liter, and the life-time of the microbial membrane was ca. 30 days at 4℃. The biosensor could specifically determine free sulfur dioxide in wine, and was not influenced by temperature or oxygen concentration. The relative standard deviations were 7.41% for red wine and 5.00% for white wine.
- 社団法人日本農芸化学会の論文
- 1993-03-23
著者
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NAKAMURA Kazuo
Department of Management and Information Systems Science Nagaoka University of Technology
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Nakamura K
Kyoto Univ. Uji Jpn
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AMANO YOSHIFUMI
Department of Fermentation Technology, Faculty of Engineering, Yamanashi University
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KUROSAWA HIROSHI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Saegusa Kengo
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Kurosawa H
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
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Saegusa Kengo
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Amano Y
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
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Amano Yoshifumi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Kurosawa Hiroshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Nakamura Kazuo
Department Of Clinical Pharmacy And Pharmacology Graduate School Of Medical And Dental Sciences Kago
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Nakamura Kazuo
Department Of Biological Sciences University Of Lethbridge
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Nakamura Kazuo
Department Of Biochemistry Kitasato University School Of Medicine
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Nakamura Kazuo
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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