A Simple Method for Forming Embryoid Body from Mouse Embryonic Stem Cells
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概要
- 論文の詳細を見る
We proposed a simple method for forming an embryoid body (EB) from mouse embryonic stem (ES) cells using a polypropylene 1.5-ml conical tube with a screw cap. An ES cell suspension containing 2 × 10^4 cells was incubated in a conical tube. After 5 d of incubation, a single EB of 440 μm average diameter was formed in the conical tube. The formation efficiency of EB, which is the ratio of the number of tubes showing EB formation to the number of tubes seeded with ES cells, was greater than 99% in the conical tube, while it was approximately 60% in a hanging drop culture. The 5-day-old EB formed by the conical tube method had a sufficient differentiation ability. The beating of the cardiac muscle was microscopically observed in the populations derived from the 5-day-old EB.
- 社団法人日本生物工学会の論文
- 2003-10-25
著者
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Kurosawa H
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
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KUROSAWA HIROSHI
Life Environment Medical Engineering, Division of Medical and Engineering Science, Interdisciplinary
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IMAMURA TETSUYA
Institute of Organ Transplants Reconstructive Medicine and Tissue Engineering, Graduate School of Me
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KOIKE MIKIKO
Life Environment Medical Engineering, Division of Medical and Engineering Science, Interdisciplinary
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SASAKI KATSUNORI
Institute of Organ Transplants Reconstructive Medicine and Tissue Engineering, Graduate School of Me
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AMANO YOSHIFUMI
Life Environment Medical Engineering, Division of Medical and Engineering Science, Interdisciplinary
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Koike Mikiko
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
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Amano Y
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
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