High Density Cultivation of Thiobacillus thiooxidans S3 in a Fermentor with Cross-Flow Filtration

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概要

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• Thiobacillus thiooxidans S3 was cultivated using thiosulfate as an energy source. The thiosulfate showed typical substrate inhibition of the cell growth of T. thiooxidans S3. It was found that the optimum thiosulfate concentration for cell growth was 20 mM. Sodium sulfate (Na_2SO_4) and potassium sulfate (K_2SO_4) which accumulated in the culture broth inhibited the thiosulfate oxidation, and thus the cell growth. Fed-batch cultivation with cross-flow filtration allowed continuous removal of inhibitory products such as Na_2SO_4 and K_2SO_4,and maintained suitable concentrations of thiosulfate in the culture broth. As a result, T. thiooxidans S3 logarithmically grew up to 7.73 g dry cell weight per liter within 96 h cultivation. The cell productivity (80.5 mg cell/l・h) was 23-fold compared with the batch cultivation.

• 社団法人日本生物工学会の論文
• 1991-07-25

著者

• NAKAMURA Kazuo

  Department of Management and Information Systems Science Nagaoka University of Technology

• Nakamura K

  Kyoto Univ. Uji Jpn

• AMANO YOSHIFUMI

  Department of Fermentation Technology, Faculty of Engineering, Yamanashi University

• KUROSAWA HIROSHI

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• NAKAGOMI TOSHIHIKO

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• KANDA KOUJI

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Nakagomi Toshihiko

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Kurosawa H

  Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin

• Kanda Kouji

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Amano Y

  Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin

• Amano Yoshifumi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Kurosawa Hiroshi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Nakamura Kazuo

  Department Of Clinical Pharmacy And Pharmacology Graduate School Of Medical And Dental Sciences Kago

• Nakamura Kazuo

  Department Of Biological Sciences University Of Lethbridge

• Nakamura Kazuo

  Department Of Biochemistry Kitasato University School Of Medicine

• Nakamura Kazuo

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

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