Microbial Sensor for the Determination of Ascorbic Acid Based on the Iron-Oxidizing Activity of Thiobacillus Ferrooxidans
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概要
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A microbial sensor consisting of immobilized Thiobacillus ferrooxidans and an oxygen electrode were prepared for the determination of ascorbic acid (AsA). A linear relationship was obtained between the sensor output and the concentration of AsA below 2.5 mM. The minimum detectable concentration of AsA was 0.1 mM. The response was reproducible with a 4.1% relative standard deviation when 1.5 mM of AsA was measured 20 times. The total time required for an assay was about 5 min. A good agreement was obtained between the microbial sensor and the 2,4-dinitrophenylhydrazine (DNP) method in the analysis of AsA in foods. Organic compounds did not affect the analysis. The activity of the microbial membrane was stable for more than 30 days.
著者
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Kato Masahiko
Department Of Allergy And Immunology Gunma Children's Medical Center
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Amano Yoshifumi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Kurosawa Hiroshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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AMANO YOSHIFUMI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Nakamura Kazuo
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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