尿中 5-hydroxyindole acetic acid (5HIAA) の螢光法による測定に関する検討

概要

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The determinations of 5HIAA in urine of rabbit were carrild out by fluorometric assay comparing with colorimetric assay. Flurometric procedure; 2 ml of urine is diluted with water to a volume of 4 ml, and to precipitate proterins 1 ml of 10% zinc sulfate is added followed by 0.5 ml of 1N NaOH. Following centrifugation of supernatant is transferred to glass-stoppered tube containg 0.3 ml of 6 NHCl, 20 ml of ether, and sufficient NaCl to insure salt saturation of the aqueous phase. The tube is shaken for 15 min. After separation of the phlase 15 ml of ether is transferred to another tube containing 3 ml of 0.5M phoshate buffer pH 7.0. After shaking, the buffer assayed with excitation at 295 mμ and fluorescence at 340 mμ by spectrophotoflurometer (Shimazu, QV-50, GF-16E) The buffer needs care to pH, 5HIAA has its own optimal conditions of pH for maximal fluorescence. The fluorometric procedure is sufficiently sensitive to measure the 5HIAA in several ml of urine, and is more discriminative and specific than colorimetric method.
日本アレルギー学会の論文
1967-07-30