アナフィラキシー・ショック時ラッテ腹腔液中の肥満細胞及び白血球の変動とヒスタミン及びセロトニン量の関連

概要

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For the purpose of investigating the change of histamine and serotonin contents in the cells in producing anaphylactic shock, mast cells and leucocytes were isolated from the peritoneal fluid of the rat by Glick's technique (1956). This technique was almost satisfactory for isolating mast cells and leucocytes. Female albino rats, weighing 150 gms, pretreated with H. pertussis vaccine were passively sensitized with anti-egg-albumin rabbit serum (antibody-titer X64) and shocked with 2% egg-albumin 24 hours after the sensitization. Histamine was extracted by Dweutsch's method and determined biologically; Serotonin was extracted and determined by Udenfriend and Weissbach's method. The results obtained were as follows; 1) Histamine and serotonin contents in peritoneal fluid were decreased after anaphylaxis. It was also demonstrated that histamine contents in mast cells remarkably decreased and serotonin decreased slightly. 2) In leucocytes histamine also decreased clearly, however, not a change of serotonin content of it was observed after anaphylaxis.
日本アレルギー学会の論文
1960-12-01