アレルギー性反応に於けるセロトニン代謝の意義についての検討 : 抗セロトニン剤, 抗アレルギー剤処置による 5-hydroxytryptophan decarboxylase 活性値の変動について

概要

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Guinea-pigs were passively sensitized with anti-egg-albumin rabbit-serum and shocked with intravenous injection of the allergen. 5-hydroxytryptophan (5-HTP) decarboxylase was extracted from kidney and liver by ammonium sulfate fractionation and purified by absorption of alumina Cγ. 5-HTP was incubated with purified 5-HTP decarboyxlase, pyridoxal phosphate and other drugs in vitro at 37℃ for 1-2 hr and the amount of increased serotonin was estimated by spectrophotoflurometric assay. The auther studied on LSD monohydrate as anti-serotonin drug, cyproheptadin, diphenylpyraline as anti-allergic drugs, and the influence of 5-HTP and 5-HTP decarboxylase activity and the following results were obtained. 1) The activity of forming serotonin by 5-HTP decarboyxlase extracted from kidney of normal guinea pigs was more powerful than that extracted from liver. 2) The activity of 5-HTP decarboxylase was remarkabely inhibited by LSD-monohydrate, but not a significant effect was observed by other drugs tested. 3) Pyridoxal phosphate used in the test as acoenzyme showed only a slight effect to anti-allergy drugs. 4) The activity of serotonin (5-HT) was found to be destructed by several anti-allergy drugs and the destruction percentage was 25% in the case of cyproheptadine, 20% in diphenylpyraline and 5% in LSD.
日本アレルギー学会の論文
1966-06-30