66. エンバクのファイトアレキシン avenanthramide 類の代謝

概要

論文の詳細を見る
Oat leaves produce avenanthramides as phytoalexin in response to infection by pathogens or treatment with elicitors. When oat leaves are floated on the elicitor solution, avenanthramides are secreted into the solution. The accumulated amounts of avenanthramides in the elicitor solution differ among avenanthramide species because of the differences in the rates of biosynthesis and metabolism. In the present study, we determined the rates of secretion and metabolism of avenanthramides using ^<13>C-labeled compounds. We administered the labeled avenanthramides into the elicitor solution, and measured the isotope-labeling ratio and total amount of each avenanthramide by ionspray LC/MS analysis. Based on the time-dependent changes in those values, we calculated the rates of secretion (v_s) and metabolism (v_m) for each avenanthramide. The v_s value of avenanthramide A was 45 nmol/h/g FW 24h after elicitor treatment, but, thereafter, no secretion was detected. The metabolism of avenanthramide A was not detected 24h after treatment, while the v_m values were around 20 nmol/h/g FW 48-96h after treatment. On the other hand, the v_s value of avenanthramide B was 131 nmol/h/g FW, and, thereafter, the value gradually decreased. The v_m values of avenanthramide B were around 60nmol/h/g FW 24-96h after treatment. These findings demonstrated that avenanthramide B was more actively secreted and metabolized than avenanthramide A. This is consistent with the substrate specificities of enzymes activities involved in the biosynthesis and metabolism of avenanthramides, which suggested active biosythesis and metabolim of avenanthramide B.
植物化学調節学会の論文
2003-10-10

66. エンバクのファイトアレキシン avenanthramide 類の代謝

スポンサーリンク

概要

著者

関連論文

スポンサーリンク