1 オオムギ抗菌性物質hordtine類の合成に関わるperoxidaseの精製と性状解析

概要

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Hordatine A, which is a dimer of p-coumaroylagmatine, occurs in barley seedlings together with hordatine B, an analogous conjugate of p-coumaroylamatine and feruloylagmatine, and hordatine M, a mixture of the glucosides of hordatine A and B. These compounds are supposed to play an important part in the resistance to fungal attacks in the juvenile stage. In the biosynthesis of hordatines, it is supposed that the last step is catalyzed by peroxidases. Since hordatines are not found in other plant species in spite of presence of p-coumarolyagmatine, the final reaction catalyzed by peroxidases is considered to be a critical step in the biosynthesis of hordatines. In this study, we found peroxidases appearing together with the occurrence of hordatines have pI (isoelectric point) values of 6.3 and 8.0. The pI 6.3 isozyme of them was purified from shoots 48 hours after seeding and characterized.
植物化学調節学会の論文
2000-11-02