C-29 トウモロコシにおける、チラミンフェルロイルトランスフェラーゼ(TFT)活性

概要

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Tyramine feruloyltransferase (TFT) catalyzes the condensation reaction to feruloyltyramine from tyramine and feruloyl-CoA. Wounding strongly induced this enzyme activity in maize leaves. TFT activity started to increase 3 hr after wounding and reached a maximum by 36 hr, being 11 times higher than the activity in control leaves. For characterization, TFT from wounded leaves was partially purified by (NH_4)_2SO_4 precipitation and two steps of anion exchage chromatography on DEAE-Sepharose and Mono Q. The best substrate was tyramine among tested amines, although phenethylamine, tryptamine and dopamine served as acyl acceptors. On the other hand, for acyl donar, feuloyl-CoA was most rapidly converted to a respective amide by TFT among hydroxycinnamoy-CoA esters.
植物化学調節学会の論文
1998-10-01