72 キトオリゴ糖及び重金属処理によるred clover(Trifolium pratense)の二次代謝産物の動態

概要

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Heavy metal ions(Au, Ag, Cu, Zn) and chitooligosaccharides((NGlc)_1-(NGlc)_6) were tested their elicitor activity in 5-day-old red clover seedlings. In roots, maackiain(Ma) and formononetin(F) were induced by the treatments with all heavy metal ions, whereas glucosyl-F(FG), malonylglucosyl-F(FGM) and malonylglucosyl-Ma(MaGM) were disappeared. On the other hand, the treatments resulted in accumulation of Ma and MaGM in shoots. Similar phenomenon were caused by the treatments with (NGlc)_4-(NGlc)_6, however no change was observed after the treatments of (NGlc)_1-(NGlc)_3. To elucidate the mechanism of accumulation of Ma and F, the enzyme activities involved in the metabolism of isoflavonoid and pterocarpan were investigated in roots. Phenylalanine ammonia-lyase activity decreased after treatments with CuCl_2 and (NGlc)_6, indicating that accumulation of Ma and F was not due to de novo synthesis. Furthermore, isoflavone glucosyltransferase activity was diminished almost completely, whereas glucosidase and isoflavone malonylesterase remained active after elicitor treatment. These results suggested that the accumulation of aglycones resulted from enzymatic degradatin of corresponding glucosides.
植物化学調節学会の論文
1999-10-01