33 オオムギにおけるジャスモン酸によるヒドロキシシンナモイルサポナリンの誘導

概要

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The effects of jasmonic acid (JA) on secondary metabolism in barley were investigated. Reversed-phase HPLC analysis revealed that the amount of a compound increased in excised barley leaves treated with JA. The compound was purified, and identified to be 6‴-feruloylsaponarin by spectroscopic analyses and alkaline hydrolysis. A related compound, 6‴-sinapoylsaponarin, was also found to accumulate in excised leaves independently of JA treatment. The accumulation of these 6‴-hydroxycinnamoylsaponarins was accompanied by a decrease of saponarin content. [8,9-^<13>C]p-Coumaric acid and [2,3,4,5,6-^2H]L-phenylalanine were effectively incorporated into the hydroxycinnamoyl moleties in 6‴-hydroxycinnamoylsaponarins, while the incorporation of the labeled precursors into saponarin part was small. These findings indicated that the hydroxycinnamoyl moieties of 6‴-hydroxycinnamoylsaponarins are de novo synthesized from phenylalanine via the phenylpropanoid pathway, and that the saponarin part is mainly provided by the constitutive saponarin pool.
植物化学調節学会の論文
2001-10-09