3 エンバクのファイトアレキシンavenanthramide類の代謝におけるペルオキシダーゼの関与

概要

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Avenanthramides, a series of substituted N-cinnamoylanthranilate derivatives, are phytoalexins in oats. The amounts of accumulated avenanthramides following elicitor treatment decreased after they reached a maximum. This accumulation was accompanied by deposition of fluorescent compounds in cell walls. In addition, the substrate specificity of the biosynthetic enzymes for avenanthramides was not necessarily correlated with composition of avenanthramides in elicitor solution. These findings suggested that avenanthramides are metabolized after their accumulation. To confirm this hypothesis, avenanthramides labeled with ^<13>C were exogenously applied into elicitor solutions. The amounts of exogenous avenanthramides decreased rapidly, and the rate of decrease was enhanced by elicitor treatment. This decreased of avenanthramides was partially inhibited by application of catalase into elicitor solution. In addition, we found that activity of peroxidase was induced by elicitor treatment in apoplastic fractions from oat leaves. These findings indicated that avenanthramides are metabolized by the action of peroxidases in oat leaves. Avenanthramides may thus be involved in the physical reinforcement of cell walls.
植物化学調節学会の論文
2000-11-02