B-22 コムギ芽生えに発現するbenzoxazinone類特異的グルコシダーゼおよびグルコシルトランスフェラーゼの精製と性状解析

概要

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Occurrence and properties of hydroxamic acids glucosyltransferase and hydroxamic acid glucosides glucosidase in wheat seedlings were investigated to elucidate their roles during juvenile stage of growth. The fashion of the appearance and disappearance of these enzyme activities were concurrent with that of hydroxamic acids. Partialy purified glucosyltransferase was more specific to DIMBOA than to DIBOA, and did not convert their precursors, HBOA and HMBOA, to their respective glucodsides.The glucosidase was suggested to be a oligomer of about 60 kD polypeptides. It showed higher specificity to DIMBOA-G than to othar hydroxamic acid glucosides. These results indicate that the occurrence of hydroxamic acids is for defense at juvenile stage, and this is regulated by these specific enzymes.
1998-10-01