5-2) MOLECULAR TOXICOLOGY ON CYTOCHROME P-450 IN HUMAN FETAL LIVERS
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概要
- 論文の詳細を見る
Among mammalian species, humans and monkeys are sole species which possess cytochrome P-450 in the liver in fetal life. Cytochrome P-450 catalyzes oxidations of a wide variety of compounds including steroids, drugs and toxicants. Thus, the purpose of this study was to examine the toxicological significance of human fetal cytochrome P-450. Firstly, we found that human fetal livers were capable of activating numbers of carcinogenic compounds. Secondly, the capacity of human fetal cytochrome P-450 to activate mutagens and carcinogens was confirmed using purified forms of cytochrome P-450. For this, we purified four forms of cytochrome P-450 from human fetal livers. Lastly, we could isolate a cDNA clone coding for P-450HFLa which is one of the major forms of cytochrome P-450 in human fetal livers. Utilizing synthesized cDNAs as probes, we found that this form of cytochrome was expressed specifically in fetuses.
- 日本トキシコロジー学会の論文
- 1991-02-28
著者
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KITADA Mitsukazu
Division of Pharmacy, Chiba University Hospital
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KAMATAKI Tetsuya
Division of Drug Metabolism, Faculty of Pharmaceuti-cal Sciences, Hokkaido University
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Kamataki T
Hokkaido Univ. Sapporo Jpn
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Kamataki Tetsuya
Department Of Pharmacology School Of Medicine Keio University:department Of Analytical Biochemistry
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Komori Masayuki
Division of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University
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Kamataki Tetsuya
Division Of Analytical Biochemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Kitada Mitsukazu
Division Of Analytical Biochemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Komori Masayuki
Division Of Analytical Biochemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Kamataki Tetsuya
Lab. Drug Metab. Fac. Pharm. Sci. Hokkaido University
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