Large-scale Production of Genetically Engineered CYP3A4 in E. coli: Application of a Jarfermenter

スポンサーリンク

概要

• 論文の詳細を見る
• 2003-04-14

著者

• FUJITA Ken-ichi

  Lab.Drug Metab., Grad.Sch.Pharm.Sci., Hokkaido Univ.

• KAMATAKI Tetsuya

  Laboratory of Drug Metabolism, Graduate School of Pharmaceutical Sciences, Hokkaido University

• NAKAYAMA Kazuo

  Laboratory of Drug Metabolism, Division of Pharmacobio-dynamics, Graduate School of Pharmaceutical S

• Fujita Ken-ichi

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Sciences Hokkaido University

• Kamataki T

  Hokkaido Univ. Sapporo Jpn

• Kamataki Tetsuya

  Department Of Pharmacology School Of Medicine Keio University:department Of Analytical Biochemistry

• Kamataki Tetsuya

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Sciences Hokkaido University

• KANAMORI Yoichi

  Laboratory of Drug Metabolism, Graduate School of Pharmaceutical Sciences, Hokkaido University

• KAWAI Hideki

  Bio Laboratory, Nihon Nosan Kogyo K. K.

• Nakayama K

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Sciences Hokkaido University

• Kamataki Tetsuya

  Division Of Analytical Biochemistry Faculty Of Pharmaceutical Sciences Hokkaido University

• Fujita Ken-ichi

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Science Hokkaido University

• Nakayama Katsuo

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Sciences Hokkaido University

• Nakayama Kazuo

  Laboratory Of Drug Metabolism Division Of Pharmacobio-dynamics Graduate School Of Pharmaceutical Sci

• Kanamori Yoichi

  Laboratory Of Drug Metabolism Graduate School Of Pharmaceutical Sciences Hokkaido University

• Kamataki Tetsuya

  Laboratory Of Drug Metabolism Division Of Pharmacobio-dynamics Graduate School Of Pharmaceutical Sci

• Kawai Hideki

  Bio Laboratory Nihon Nosan Kogyo K. K.

• Kamataki Tetsuya

  Lab. Drug Metab. Fac. Pharm. Sci. Hokkaido University

関連論文

• MACROMOLECULAR BINDING OF CARCINOGENIC/MUTAGENIC N- HYDROXYARYLAMINES THROUGH O-ACETYLATION PATHWAY
• MECHANISM OF MUTATION AND METABOLIC ACTIVATION OF NITRO-, NITROSO-, HYDROXYLAMINO-COMPOUNDS IN NITRO-COMPOUNDS RESISTANT MUTANTS OF AMES TESTAR STRAIN (TA98NR, TA98/1,8-DNP_6)
• Two Novel Single Nucleotide Polymorphisms (SNPs) of the FMO3 Gene in Japanese
• A Mutation in the Flavin-containing Monooxygenase 3 Gene and its Effects on Catalytic Activity for N-oxidation of Trimethylamine In Vitro
• Overexpression of CYP2A6 in human colorectal tumors
• O11-01 Induction of Human CYP1A1 by 2-Phenyl-benzotriazoles(PBTAs)which Are Metabolically Activated by CYP1A1
• O-18 Role of Human N-Acetyltransferase in the Metabolic Activation of 2-Phenylbenzotriazole (PBTA) Derivatives.(Proceedings of the 27th Annual Meeting)
• 21C-01-1 Metabolic Activation of PBTA-1 and PBTA-2 by Human Cytochrome P450 1A1.
• Cytochrome P450 gene expression levels in peripheral blood mononuclear cells in comparison with the liver
• Establishment of in silico systems to predict the effects of genetic polymorphism on the enzymatic activity of cytochrome P450
• ROLE OF GROWTH HORMONE ON THE EXPRESSION OF SEX-SPECIFIC FORMS OF CYTOCHROME P-450,P-450-MALE AND P-450-FEMALE
• STUDIES ON SEX-SPECIFIC FORMS OF CYTOCHROME P-450 IN MALE AND FEMALE RATS
• Inhibitory Effects of Nicardipine to Cytochrome P450 (CYP) in Human Liver Microsomes(Biopharmacy)
• Co-operative Regulation of the Transcription of Human Dihydrodiol Dehydrogenase(DD)4/Aldo-Keot Reductase(AKR)1C4 Gene by Hepatocyte Nuclear Factor(HNF)-4α/γand HNF-1α.
• Characterization of a Novel Variant (S145C/L311V) of 3α-Hydroxysteroid/Dihydrodiol Dehydrogenase in Human Liver.
• Factor(s) Regulating the Induction of CYP1A1 by Aryl Hydrocarbons in Rabbits
• CHARACTERIZATION OF TRANSGENIC MICE CARRYING A HUMAN FETUS-SPECIFIC CYP3A7.
• "B-9 Factors Regulating CYP1A1 Induction in Adult Rabbit Livers.
• Expression and Characterization of Dog CYP2D15 Using Baculovirus Expression System^1
• Inhibiton by EGCG of benzo[a]pyrene-induced mutations in the lung of HITEC mice.
• CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers.(GENERAL SESSION BY ORAL PRESENTATION)(DRUG METABOLISM)
• AHR-mediated suppression of PPARα target gene expression caused by exposed polycyclic aromatic hydrocarbons (TOXICOGENOMICS AND TOXICOPROTEOMICS) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
• SUPPRESSION OF PPAR ALPHA SIGNAL CAUSED BY POLYCYCLIC AROMATIC HYDROCARBONS.(GENERAL SESSION BY POSTER PRESENTATION)(MOLECULAR TOXICITY & TOXICOGENOMICS)
• 2B-08 Establishment of Genetically Engineered E.coli Highly Sensitive to Nitrosamines by Introducing Human CYP2E1
• Twenty One Novel Single Nucleotide Polymorphisms (SNPs) of the CYP2A6 Gene in Japanese and Caucasians
• CYP3A5 Contributes Significantly to CYP3A-mediated Drug Oxidations in Liver Microsomes from Japanese Subjects
• プロポフォール薬物動態と心拍出量
• TOXICOLOGICAL SIGNIFICANCE OF A FORM OF CYTOCHROME P-450 HIGHLY PURIFIED FROM LIVER MICROSOMES OF PCBTREATED RATS : CATALYTIC AND IMMUNOCHEMICAL EXAMINATIONS OF A HIGH SPIN FORM OF CYTOCHROME P-448
• The Role of Glutathione in the Activation and Detoxication of a Naturally Occurring Mutagen-Carcinogen, Trp-P-2 (Regular Presentations) (Proceedings of the 10 th Symposium on Environmental Pollutants and Toxicology)
• MECHANISMS INVOLVED IN THE COVALENT BINDING OF A TRYPTOPHAN-PYROLYSATE PROMUTAGEN, TRP-P-2,TO DNA
• Metabolic activation of amino acid-pyrolysates and its relation to mutagenicity
• STUDIES ON CYTOCHROME P-450 RESPONSIBLE FOR THE OCCURRENCE OF SEX DIFFERENCE OF DRUG METABOLISM IN THE RAT
• A SENSITIVE METHOD FOR THE DETERMINATION OF BIPHENYL HYDROXYLASE ACTIVITY AND IT'S TOXICOLOGICAL APPLICATION
• MECHANISMS OF METABOLIC ACTIVATION OF A TRYPTOPHAN PYROLYSATE, 3-AMINO-1-METHYL-5H-PYRIDO [4,3-b] INDOLE
• PURIFICATION AND PROPERTIES OF CYTOCHROME P-450 AND NADPH-CYTOCHROME C (P-450) REDUCTASE FROM MICROSOMES OF HUMAN LIVERS
• STUDIES ON THE METABOLIC ACTIVATION AND THE MECHANISM OF TOXICITY OF DRUGS V. REDUCTION OF N-HYDROXY DERIVATIVE OF AN AROMATIC AMINE, 2-ACETYLAMINOFLUORENE BY CYTOCHROME P-450 (The 6th Meeting for the Study of Toxic Effect)
• DEGRADATION OF CYTOCHROME P-450 ASSOCIATED WITH METABOLIC ACTIVATION OF CARBON TETRACHLORIDE (The 5th Meeting for the Study of Toxic Effect)
• Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a
• Expression of human CYP1A2 capable of activating heterocyclic amine in Bm 6 cells using baculovirus (BmNPV) system.
• In vivo Evaluation of Coumarin and Nicotine as Probe Drugs to Predict the Metabolic Capacity of CYP2A6 Due to Genetic Polymorphism in Thais
• Novel Nonsynonymous Polymorphisms of the CYP1A1 Gene in Japanese
• Toxicological roles of human and rodent CYP2As in the mutagenic activation of N-nitrosamines.(GENERAL SESSION BY ORAL PRESENTATION)(DRUG METABOLISM)
• Novel Mutations of the CYP2A6 Gene in a Thai Population with Lowered Capacity of Coumarin 7-Hydroxylation
• O11-15 Mutagenic activation of tobacco-related N-nitrosamines by rat CYP2As in comparison with human CYP2A6.
• O11-14 Comparison of the toxicolqical roles of mouse CYP2A with human CYP2A6.
• Genetic Polymorphism in the 5'-Flanking Region of Human CYP1A2 Gene : Effect on the CYP1A2 Inducibility in Humans
• Two Novel Haplotypes of CYP2D6 Gene in a Japanese Population
• Eighteen Novel Polymorphisms of the CYP2A13 Gene in Japanesse
• METABOLIC ACTIVATION OF AFLATOXIN B_1 BY HUMAN PLACENTAL MICROSOMES
• Formation of an Antibacterial Metabolite from a New Macrolide Compound 23-O-Benzyl-5-mycaminosyl-tylonolide (TMC-101), by a Hepatic Microsomal Drug-Metabolizing Enzyme System
• 5-2) MOLECULAR TOXICOLOGY ON CYTOCHROME P-450 IN HUMAN FETAL LIVERS
• Immunochemical Studies for the Presence of P-450 HFLa, a Form of Cytochrome P-450 in Human Fetal Livers in Human Hepatocellular Carcinoma Cells
• SPECIES DIFFERENCE OF CYTOCHROME (S) P-450 CROSS-REACTIVE WITH ANTI-P-450-MALE ANTIBODIES
• Large-scale Production of Genetically Engineered CYP3A4 in E. coli: Application of a Jarfermenter
• 21C-04-1 Inhibition of the Activity of Human Cytochrome P450 Involved in the Metabolic Activation of Procarcinogens by Green Tea Catechins.
• Establishment of Salmonella Tester Strains Co-expressing Cytochrome P450 and NADPH-cytochrome P450 Reductase.
• Application of Genetically Engineered Bacteria Carrying Human Cytochrome P450 to Toxicological Studies
• Metabolic Activation of Dimethylnitrosamine(DMN) by Human CYP2E1 Expressed in O^6-Methylguanine-DNA Methyltransferase-Deficient Cells
• Establishment Of A Salmonella Tester Strain Ultra-Sensitive To Mutagenic Heterocyclic Amines
• DEVELOPMENT OF PRACTICAL METHODS WHICH MIMIC DRUG METABOLISM IN HUMANS.
• EXPRESSION OF RECOMBINANT HUMAN CYTOCHROME P450 (P450 OR CYP) 2A6 IN ESCHERICHIA COLI.
• "S2-3 STRATEGIC PROPOSALS TO AVOID TOXIC DRUG INTERACTIONS DURING NEW DRUG DEVELOPMENT : MOLECULAR TOXICOLOGY PROPOSALS (Strategy how we can learn drug-drug interaction during the development of a new drug)
• Stable expression of CYP3A4 and CYP3A7, adult and fetal-specific forms of cytochrome P450 in human liver, in CHL cells and its application to toxicological testing.
• Effects of NO-1886 (Ibrolipim), a Lipoprotein Lipase-Promoting Agent, on Gene Induction of Cytochrome P450s, Carboxylesterases, and Sulfotransferases in Primary Cultures of Human Hepatocytes
• Detection of Three Genetic Polymorphisms in the 5'-Flanking Region and Intron 1 of Human CYP1A2 in the Japanese Population
• Homologous Unequal Cross-Over within the Human CYP2A Gene Cluster as a Mechanism for the Deletion of the Entire CYP2A6 Gene Associated with the Poor Metabolizer Phenotype
• Thiopurine Methyltransferase Genotype and Phenotype Status in Japanese Patients with Systemic Lupus Erythematosus(Biopharmacy)
• Association of CYP2A6 Gene Deletion with Cigarette Smoking Status in Japanese Adults
• 221 INTRODUCTION OF CYTOCHROME P-450 cDNAS INTO CULTURED MAMMALIAN CELLS AND THEIR APPLICATION TO IN VITRO TOXICOLOGICAL STUDIES
• Genotyping of Thiopurine Methyltransferase Using Pyrosequencing^(Biopharmacy)
• Studies on the Interactions between Drug and Estrogen. II. On the Inhibitory Effect of 29 Drugs Reported to Induce Gynecomastia on the Oxidation of Estradiol at C-2 or C-17
• Preventive Effects of Hange-shashin-to on Irinotecan Hydrochloride-Caused Diarrhea and Its Relevance to the Colonic Prostaglandin E_2 and Water Absorption in the Rat
• Production of Inhibitory Polyclonal Antibodies against Cytochrome P450s
• Protective Effects of Kampo Medicines and Baicalin against Intestinal Toxicity of a New Anticancer Camptothecin Derivative, Irinotecan Hydrochloride (CPT-11), in Rats
• W1-2 Toxicological Significance of Genetic Polymorphism of Cytochrome P450 : Genetic Polymorphism of CYP2A6 and Lung Cancer Risk.(Proceedings of the 27th Annual Meeting)
• 21C-01-2 Relationship Between Genetic Polymorphism of CYP2A6 and Lung Cancer Risk.
• Separation of microsomal cytochrome b_5 via phase separation in a mixed solution of Triton X-114 and charged dextran
• Selective Solubilization of Microsomal Electron-Transfer Proteins with Alkylglucoside
• 21WA-04-2 Regulation of NAD(P)H: quinone oxidoreductase_1 (NQO_1) Gene Expression in Guinea Pig.
• Metabolism of Selegiline Hydrochloride, a Selective Monoamine B-type Inhibitor, in Human Liver Microsomes
• Determination of Isepamicin in Human Plasma by HPLC with Fluorescence Detection after Derivatization Using 6-Aminoquinolyl-N-hydroxysuccinimidyl-carbamate(Analytical Biochemistry)
• A novel intronic mutation that may affect genotyping result of CYP2C8 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is strongly associated with CYP2C8^*3 in a South American population
• Time-Dependent Induction of Midazolam-1-hydroxylation Enzymes in Rats Treated with St. John's Wort(Biopharmacy)
• OCCURRENCE OF AUTOANTIBODY TO PROTEIN DISULFIDE ISOMERASE IN PATIENTS WITH HEPATIC DISORDER
• OCCURRENCE OF AUTOANTIBODY TO PROTEIN DISULFIDE ISOMERASE IN RATS WITH XENOBIOTIC-INDUCED HEPATITIS
• Relevance of Irinotecan Hydrochloride-Induced Diarrhea to the Level of Prostaglandin E_2 and Water Absorption of Large Intestine in Rats
• MOLECULAR CLONING OF MOUSE LIVER FLAVIN CONTAINING MONOOXYGENASE (FMO1) cDNA AND CHARACTERIZATION OF THE EXPRESSION PRODUCT : METABOLISM OF THE NEUROTOXIN, 1,2,3,4- TETRAHYDROISOQUINOLINE (TIQ)
• Association of CYP2A6 Gene Deletion with Cigarette Smoking Status in Japanese Adults
• HIGH SUSCEPTIBILITY TO AFLATOXIN B_1 AND BENZO[A]PYRENE OF BALB3T3 A31-1-1 CELLS EXPRESSING MONKEY CYP1A1
• LOW ACTIVITY OF ALDEHYDE DEHYDROGENASE (ALDH) IN LIVER OF SUNCUS MURlNUS : POSSIBLE EXPLANATION FOR HIGH SENSITIVITY TO ALCOHOL
• Inhibition of UDP-glucuronosyltransferase by Aglycons of Natural Glucuronides in Kampo Medicines Using SN-38 as a Substrate
• INHIBITION BY SKF 525-A OF 7-ETHOXYCOUMARIN O-DEETHYLATION IN MICROSOMAL AND THE RECONSTITUTED MONOOXYGENASE SYSTEMS FROM PCB-TREATED RAT LIVERS
• O-17 Role of Human Cytochrome P450 in the Metabolic Activation of N-Alkylnitrosamines.(Proceedings of the 27th Annual Meeting)
• A Major Genotype in UDP-glucuronosyltransferase 2B15
• Genetically Engineered Bacterial Cells Co-expressing Human Cytochrome P450 with NADPH-cytochrome P450 Reductase : Prediction of Metabolism and Toxicity of Drugs in Humans
• Cell-specific transcriptional regulation of the human CYP1B1 (DRUG METABOLISM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
• Lung tumorigenesis promoted by anti-apoptotic effects of cotinine, a nicotine metabolite through activation of PI3K/Akt pathway
• Interspecies homology of cytochrome P-450: Inhibition by anti-P-450-Male antibodies of testosterone hydroxylases in liver microsomes from various animal species including man.

もっと見る 閉じる

スポンサーリンク