水稲下位節間の挫折抵抗力に関する組織形態学的研究 : 第2報下位節間の2次壁肥厚過程に関する光学および電子顕微鏡観察

概要

論文の詳細を見る
In this paper, we report an observation with light and electron microscope on the process of the secondary wall thickening and lignin accumulation in the cells of the 4th internode from the top of rice culm, var. Koshihikari. Materials for ultra thin sections were fixed in 2-4% glutaraldehyde, postfixed with 1-2% osmium tetroxide, and embedded in Spurr's resin. Meshes were stained with uranium and lead. For histochemical detections of lignin, free hand sections, 60-80 μm in thickness, were treated with Maule's reagents (M-reaction) and phloroglucinol-HCl (P-reaction). The secondary wall of the cortical fibers makes a start of thickening just after the cell has completed its elongation. It usually consists of three layers; S_1, S_2 and S_3. In crease in secondary wall thickness is associated with increase in number of Golgi apparatus, Golgi vesicles, exocytosis and invagination. The ribosomes are often present as polysomes on the rough endoplasmic reticulum, some of which connect with the plasmalemma (Figs. 1-4). The fiber cells have smaller diameter and thicker walls in the outer rows near the epidermis than those in the inner rows which sometimes lack S_3 layer. The secondary walls of the tips of fiber cells thicken more slowly and are thinner than those of the central part (Fig. 5). The epidermal long cells have a large number of small papillae, and a number of large ones on both sides of the short cells, but a fewer papillae on the small vascular bundle (Fig. 6). The aspects of cell organelles at their wall thickening stage are similar to those in the cortical fibers (Figs. 8-10). At this stage, silica deposition cetripetally progresses from the inside of cuticular layer in all the outer walls of the long cells, especially in those of the silica cells (Figs. 9-12). As for the parenchyma cells, a large vacuole is formed from an early stage of cell development, and conspicuous invaginations are often seen accompanying with ribosomes (Fig. 13). No lignin is detected in elongating cell walls except those of the protoxylem vesseles. On and after beginning of the secondary wall thickening, M-reaction begins by appearing (Fig. 14A). On and after beginning of the S_2 layer thickening, P-reaction follows up the traces of M-reaction. M-reaction decreases in the part where P-reaction is positive (Fig. 14B). In the 4th mature internode, P-reaction is generally intensive in thc epidermis, cortical fibers and small vascular bundle. M-reaction is thc most intensive in the large vascular bundle sheath (Fig. 14C). In a fiber cell, P-reaction is the most intensive in the middle lamella-primary wall complex and decreases centripetally. On the contrary, M-reaction is intensive on the faint parts of P-reaction, but both reactions are not obvious in the parenchyma. In briefly saying, P- and M-reactions relate complementally each other. The secondary wall thickening and the lignin accumulation in the 4th internode of the cultivar terminate about 5 days after heading and entirely complete after next 10 days.
日本作物学会の論文
1983-03-05

水稲下位節間の挫折抵抗力に関する組織形態学的研究 : 第2報下位節間の2次壁肥厚過程に関する光学および電子顕微鏡観察

スポンサーリンク

概要

著者

関連論文

スポンサーリンク

水稲下位節間の挫折抵抗力に関する組織形態学的研究 : 第2報 下位節間の2次壁肥厚過程に関する光学および電子顕微鏡観察

スポンサーリンク

概要

著者

関連論文

スポンサーリンク

水稲下位節間の挫折抵抗力に関する組織形態学的研究 : 第2報下位節間の2次壁肥厚過程に関する光学および電子顕微鏡観察