9 4-nitroquinoline 1-oxide刺激ラット舌粘膜上皮細胞の培養とin vitroでの癌化(第491回大阪歯科学会例会)

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We obtained epithelial cells from the tongue of rats treated with 4-nitroquinoline 1-oxide and transplanted cells that had been subcultured 20 times in the subcutaneous tissue of nude mice. A firm tumor was formed, that was histologically confirmed to be squamous cell carcinoma. We observed telomerase activity, TERT, c-Myc, p 16, and p 53 expression in these cells during the process of carcinogenesis. We also examined β-galactosidase expression. The results showed that telomerase activity and expression of TERT mRNA increased with the number of subcultures. Although p 16 expression also increased, a sharp decrease was obsearved after 20 subcultures. While the control cells showed strong β-galactosidase expression, this was not observed in all cells. We concluded that carcinogenesis had a direct effect on the increase in telomerase activitv and on the decrease in cellular senescence caused by p 16.
大阪歯科学会の論文
2004-03-25

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