10 口腔由来偏性嫌気性グラム陰性桿菌が保有するβ-lactamase遺伝子に関する研究(第511回大阪歯科学会例会)

概要

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We studied 12 strains of 6 obligate anaerobic Gram-nagative rods (OGNR) isolated from the saliva of adult humans for elucidation of the β-lactam antibiotic-resistant mechanism in odontogenic infectious diseases. DNA polymerase chain reaction (DNA-PCR) sequencing of the OGNR was performed with designed primers and compared with data from the DNA Data Bank of Japan (DDBJ). The PCR products were further induced for gene-transformation in E-coli. A 922-944 bp PCR product showing homology with the known β-lactamase gene was acquired in the specimens sequenced with CfxA 2 primers. Certain PCR products were also observed in other primers. Although all the PCR products were transformed in E-coli, β-lactamase productivity was confirmed only with selective culture media. These results show that the β-lactamase gene is not specific in oral OGNRs. However, although the gene is widely found in oral OGNRs, it is usually not expressed in OGNR strains isolated from areas other than the oral cavity.
大阪歯科学会の論文
2008-03-25