歯髄刺激による三叉神経脊髄路核尾側亜核のFos蛋白と皮膚抑制(鍼灸基礎)

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We have thought that widespread cutaneous inhibition, which is induced by coniditining-stimulation of remote skin area, might involve in the mechanisms underlying acuouncture analgesia. For example, human blink reflex or rat jaw opening reflex (JOR) is known to be inhibited by an acupuncture-like single pulse conditioning-stimulation (CS) delievered to hand or foot, prior to an electric test-stimulation (TS) evoking the reflex. On the other hand, the c-fos, immediate-early gene, has been shown to express by noxious stimulation. Therefore, we immunohistochemically investigated whether or not CS suppressed the expression of c-fos in rat spinal trigeminal nucleus (STN). Adult rats were used in this study (n=2) JOR was evoked by a TS to unilateral tooth pulp under urethane anesthesia and was recorded by means of EMG. Animals in group I received only TS (0.2 Hz) for 30min (n=4). A cmbination with CS and TS was deliverd to rats in group II (n=4). Fifty milli seconds before each TS,CS was applied to ipsilateral sciatic nereve of rats. Control rats were undergone sham operation to compare with group I or II (n=4). Thirty minutes following the treatment, all rats were perfused with cold Zamboni's fixative, and then c-fos protein (Fos)-like structures were immunohistochemically detected in STN. In the control group,the exPression of Fos-like immunoreactive (IR) cells evoked by sham operation was observed in the superficial layer of bilateral STN caudalis. The number of ipsilateral Fos-IR cells in group I Was significantly increased as compared with that in control one (l.6fold). On the other hand, we found no difference between the number of these cells in group II and in the control group. Each CS inhibited the magnitude of EMG during the stimulation. These results suggest that the expression of Fos-like protein by TS is suppressed by CS in rat STN・
関西鍼灸大学の論文
1993-04-01

歯髄刺激による三叉神経脊髄路核尾側亜核のFos蛋白と皮膚抑制(鍼灸基礎)

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