ハリ通電刺激により視床下部-下垂体系に発現するfos蛋白(鍼灸基礎)

概要

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Acupuncture is generally thought to give the remedial value partially through endocrine system. while the mechanism is still unclear. In this study, we investigated whether or not electro-acupuncture (A-E) responded to hypothalamo-hypophysial system by immunohistochemical methods using an antibody for c-fos protein (Fos) in the rat. C-fos, an immediate-early gene. has been shown to express in the spinal dorsal horn, brain stem, and forebrain in response to noxious stimulation and stress. Under ketamine anesthesia, the nalms of the unilateral hindlimb were treated with high voltage (about 5-10 v) 5 Hz E-A (pulse of 0.1 ms) for 30 min. One, three and five hours after beginning of E-A, respectively, Fos-like structures were immunohistochemically detected. 0ne hour after E-A, Fos-like immune-reactivity (Fos-IR) was detected in the anterior lobe, but not in the middle and the posterior lobe of hypohysis. Fos-IR neurons in theanterior lobe were also observed 3 hours after E-A, but rarely 5 hours after. Moreover, Fos-IR was displayed in some of neurons in the hypothalmic paraventricular, supraoptic, suprachiasmatic nuclei etc. None or faint Fos-IR was seen in these areas of untreated rats. These findings suggest that E-A is able to cause the significant response in the activity of hypothalamo-hypophysial system.
関西鍼灸大学の論文
1994-04-01