ブラジキニンに対するマウス脊髄後根神経節培養細胞の興奮性

概要

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It is important to identify primary nociceptive neurons in cultured cells when studying the neuronal pain mechanism with cultured dorsal root ganglion (DRG) cells. In the case of primary culture of DRG, there were large-sized cells (>30μm) derived from A-fiber and small-sized cells (<25μm) derived from C-fiber. We studied how cells can be classified according to their response to, algesic agents, bradykinin (BK), prostaglandin E_2 (PGE_2), and capsaicin (CAP). BK application (10μM) to a small-sized cell (<25μm) evoked action potential in the cell's body (soma). PGE_2 (20μM) or CAP (2μM) also induced action potential in small-sized cells. The large-sized cells (>30μm) could not be excited by application of BK or PGE_2. In the case of medium-sized cells (25-30μm), there were both no-respond cells, and respond-cells to application of algesic agents. The no-respond cells are probably other sensory cells. These results suggest that a small-sized cell (<25μm) is a primary nociceptive neuron derived from C-fiber, and is useful in studying the pain-mechanism.
東海大学の論文
1997-03-30