酵素サイクリングセンサによる生体成分の測定

概要

論文の詳細を見る
Enzyme sensors for determining ammonia and γ-aminobutylate are described. An enzyme electrode with NAD cycling for ammonia analysis was composed of an immobilized multienzyme (NAD synthetase, alcohol dehydrogenase, and NADH oxidase) membrane and a hydrogen peroxide electrode. The dynamic range of the sensor was 0.2〜40μM for ammonia. Another type of enzyme electrode with NADP cycling for γ-aminobutylate was constructed by combining an immobilized multienzyme (γ-amynobutylate ketoglutarate aminotransferase, succinic semialdehyde dehydrogenase, and NADPH oxidase) membrane with a hydrogen peroxide electrode. The dynamic range of the sensor was 0.1〜5mM for γ-aminobutylate. The response time of the sensors was about one minute. The present enzyme sensor method offers a rapid, simple and precise analytical procedure for the determination of biocomponents in body fluid and food.
東海大学の論文
1999-03-30