バイオセンサによる動物細胞培養プロセスの制御

概要

論文の詳細を見る
Biosensors were applied to monitor the concentration of glucose, lactate and glutamine in a culture medium during the production of human erithropoietin by the cultivation of mouse C127 transformants. The biosensors were constructed by assembling polalographic electrodes for sensing hydrogen peroxide, corresponding immobilized enzyme membranes and separation membranes. Glucose oxidase, lactate oxidase, and glutaminase-glutamate oxidase were utilized for the determination of glucose, lactate and glutamine, respectively. Based upon the evaluation of the time course of glutamine in culture medium, a suitable composition of a culture medium was developed for the high density culture of the transformants. The measured values of glucose and lactate on periodic samples drawn from the culture system were utilized to control lactate and glucose levels by the media diluction ratio and glucose feed rate. The high productivity of erithropoietin for 140 days in a continuous culture was attained by the monitoring of the medium components using biosensors.
東海大学の論文
1995-03-30