キトサン膜への複合酵素系の固定化とバイオセンサへの応用

概要

論文の詳細を見る
Bienzyme biosensors, in which two different types of enzymes are utilized, were developed for the determination of NAD, γ-aminobutylate (GABA) and acetylcholine. The biosensors were constructed by combining immobilized bienzyme membranes with electrodes sensing oxygen or hydorogen peroxide. Alcohol dehydrogenase・NADH oxidase, gabase・glutamateoxidase, and acetylcholine esterase・choline oxidase were utilized for NAD sensor, GABA sensor and acetylcholine sensor, respectively. These enzymes were immobilized onto a porous membrane by entrapping with chitosan gel. The response time of the sensors was about one minute. The dynamic ranges of the sensors were 5μM〜1mM for NAD, 0.25〜5mM for GABA and 15μM〜0.4mM for acetylcholine. The present biosensor method offers a rapid, simple and precise analytical procedure for the determination of biocomponents in body fluid and food.
東海大学の論文
1998-03-30