バイオセンサによる生体試料中の尿素の定量

概要

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An enzyme electrode suitable for the determination of urea in biological samples like blood and artificial kidney dialysate is described. The enzyme urease was immobilized between two types of porous membranes by a gultaraldehyde crosslinking method : A polycarbonate membrane which is permeable by urea and water, and a poly (tetrafluoroethylene) membrane which is permeabile by ammonia gas. The enzyme electrode for urea determination was constructed by combining the immobilized urease membrane with a pH electrode. The urea sensor responded to urea in concentrations over 10-200mg/dl. The response time was 30 seconds in a flow-injection method. When the urea sensor was applied for the determination of urea in serum, within-day precision (CV), analytical recovery, and correlation coefficient between the sensor method and the conventional enzyme colorimetric method were 7.4% with a mean value of 28.1mg/dl, 98%, and 0.988, respectively. The urea sensor was also applied for the monitoring of urea concentration in dialysate for hemodialysis.
東海大学の論文
1994-03-30