モルモット盲腸筋層間神経叢培養グリア細胞の受容体、イオンチャネル及び膜電位に対する神経伝達物質の作用

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The electrophysiological properties and the role of the glial cells obtained from an explant culture of the myentericplexus of the guinea-pig cecum were studied using the patch-clamp technique. The myenteric glial cells were identifiedusing fluorescent antibodies, anti-S100 and anti-GFAP. A polygonal shaped-cell was used for the patch-clamp recording.The cell membrane of the glial cells located in the cecum myenteric plexus had high- and low-conductance Cl-channelswhich were insensitive to Ca^2^+ increases. Adrenalin or forskolin application evoked the opening of the high- and lowconductanceCl^-channels; glutamic acid evoked the opening of the low-conductance Cl ^− channel. The glial cells possesseda Ca^2^+- and voltage-activated K^+ selective channel that was evoked by ACh or ATP application. The membrane potentialresponse data showed that ACh evoked hyperpolarization of the cell via a muscarinic receptor pathway, and ATP evokedhyperpolarization of the cell via a P_2 purinoceptor pathway. An adrenalin-evoked response was involved in a β -adrenergicreceptor pathway, and a glutamic-evoked response was involved in a non NMDA-receptor pathway. These results suggestthat autonomic nervous system transmitters, ACh and adrenalin, may have a reciprocal action in the alimentary tract viareceptor, ion channel, and membrane potential changes, and that ATP and glutamic acid also may have a similar reciprocalaction in the alimentary tract.
2010-03-31