培養条件による腸管神経節ニューロンとグリア細胞の形態と電気的性質の変化

概要

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We used an explant culture of myenteric ganglion from Auerbach's plexus of guinea-pig cecum and studied the electrical property of neurons and glial cells using a patch-clamp technique. Many networks, a few networks and no networks between neurons from a 14-day-old culture were seen in a culture medium using a 20% (volume %) fetal bovine serum (FBS), a 10% FBS and a 5% FBS, respectively. The success percentages of patch-sealing between patch-pipette and cell membrane were 33% for 10% FBS and 23% for 20% FBS in neurons, and 46% for 10% FBS and 33% for 20% FBS in glial cells. The membrane potentials were -41mV (mean value) for 10% FBS and -55mV for 20% FBS in neurons, and -30mV for 10% FBS and -36mV for 20% FBS in glial cells. These results suggest that the cells from explant culture using a 20% FBS would be useful to study the network of the neurons by the patch-clamp technique.
東海大学の論文
2000-03-10