SHRSP大動脈中膜のエラスチン含量 : 色度座標法による検討(A 基礎的研究)(I 発生病理)

概要

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The elastin content of the media of SHRSP aorta was measured with a microscopic spectro-photometer, and analyzed by application of the chromaticity coordinate systems. The spectral transmittance values of the Weigert-stained elastic fibers were processed to specify the stained histological color with the aid of the x, y and Y' coordinate system. The coloration of the elastic fibers as well as the inter-lamellar space was characteristic in the color space, respectively. The relative concentration of the chromophore representing elastin was calculated by comparison of the Y'-values with that of the bovine elastin. The elastin concentration within a minute volume corresponding to the smallest scanning spot was calculated by the use of the bovine nuchal ligament unit (BNLU). The volume percent which is representing the relative elastin volume within the aortic (or arterial) media was obtained by the use of the scanning method with a minute spot. The product of the volume percent with the relative chromophore concentration thus obtained is one of the useful indices for the elastin content of vessels. The elastin content among serial preparations was discussed with relation to the estimated experimental errors.
近畿大学の論文
1980-09-25