Lobular Difference in Fibrotic Changes in Rat Cirrhosis Model Induced by Carbon Tetrachloride
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概要
- 論文の詳細を見る
The rat liver cirrhosis induced by carbon tetrachloride (CCl4) animal model is widely used in efficacy studies. In the present study, we examined the difference among hepatic lobes of fibrotic lesions in CCl4-induced liver cirrhosis rat models. Male Wistar rats were given phenobarbital which was mixed into their drinking water. CCl4 was orally administered to rats weekly for 9 weeks. The total dosage of CCl4 was 1.48 ml to 1.68 ml per animal. At necropsy, the liver was divided into lobes: left lobe, median lobe, right lobe, caudate lobe, and papillary process, and the weight of each lobe was measured. Hepatic lobes were submitted to histopathological examination, morphometric analysis of fiber composition content and hydroxyproline content. The weight ratio of lobe to whole liver markedly decreased in the median lobe and right lobe, whereas the weight ratio of lobe to whole liver of the left lobe, papillary process, and caudate lobe increased. In the median and right lobes, strong fibrotic lesions and distinct micronodular formation were observed. In the other lobes, only weak fibrotic lesions were observed. Additionally, high values in fiber composition content were observed in the median and right lobes compared with the other lobes. Hydroxyproline was also markedly higher in the median lobe than in the left lobe. These findings suggest that lobular differences in fibrotic lesions are present in CCl4-induced liver cirrhosis rat models, and that severe fibrotic lesions are induced in the median and right lobes.
- 日本毒性病理学会の論文
- 2004-12-30
著者
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鈴木 雅実
Safety Assessment Dep. Fuji Gotemba Res. Laboratories Chugai Pharmaceutical Co. Ltd.
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鈴木 雅実
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Fujii Etsuko
中外製薬富士御殿場研究所
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Fujii Etsuko
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Fujii Etsuko
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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Fujii Etsuko
Department Of Veterinary Pathology Faculty Of Agriculture Iwate University
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SUZUKI Masami
Department of Safety Assessment, Fuji Gotemba Research Laboratory, Chugai Pharmaceutical Co., Ltd.
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杉本 哲朗
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Sugimoto Tetsuro
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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Sakurai Takayuki
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Ogawa Yumie
Fuji Gotemba Research Labs. Chugai Pharm. Co. Ltd.
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鈴木 雅実
Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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YOROZU Keigo
Product Research Department, Kamakura Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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TERUYA Shino
Department of Toxicology, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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OGAWA Yumie
Department of Toxicology, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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ITO Hirotaka
Genome Antibody Product Research Department, Tsukuba Research Laboratories, Chugai Pharmaceutical Co
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SUGIMOTO Tetsuro
Department of Toxicology, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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YOROZU Keigo
Department of Toxicology, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd.
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Sugimoto Tetsuro
Department Of Pathology Keio University School Of Medicine
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Teruya Shino
Department Of Toxicology Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Ito Hirotaka
Genome Antibody Product Research Department Tsukuba Research Laboratories Chugai Pharmaceutical Co.
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Sugimoto Tetsuro
Safety Assessment Laboratory Chugai Pharmaceutical Co. Ltd.
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Suzuki Masami
Safety Assessment Department Chugai Pharmaceutical Co. Ltd.
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Ogawa Yukio
Fuji Gotemba Research Labs. Chugai Pharm. Co. Ltd.
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FUJII Etsuko
PharmaLogicals Research Pte. Ltd.
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Yorozu Keigo
Department Of Toxicology Fuji Gotemba Research Laboratories Chugai Pharmaceutical Co. Ltd.
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Yorozu Keigo
Product Research Department Chugai Pharmaceutical Co. Ltd.
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Suzuki Masami
Department Of Metallurgy
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Suzuki Masami
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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SUZUKI Masami
Department of Agricultural Chemistry, Gifu University
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